Abstract
BackgroundTo investigate the function of miR‐191‐5p in lung adenocarcinoma and its possible mechanism.MethodsQRT‐PCR was adopted for the detection of the expression levels of miR‐191‐5p and SATB1 (HGNC: 10541). The effects of miR‐191‐5p and SATB1 on cell proliferation and migration were examined through the CCK‐8 and Transwell assays. Subsequently, the binding relationships between miR‐191‐5p and SATB1 were confirmed by dual‐luciferase reporter gene assay. Finally, the potential mechanisms of action of miR‐191‐5p were explored through a serious of in vivo and in vitro experiments.ResultsLung adenocarcinoma patients had a notably lower expression level of miR‐191‐5p than controls, patients with metastasis had a lower level than those without metastasis, and the level in patients with lung adenocarcinoma in stage III‐IV was lower than that in patients with lung adenocarcinoma in stage I‐II. Overexpression of miR‐191‐5p repressed the migration and proliferation of lung cancer A549/H1650 cells. According to the reporter gene assay, miR‐191‐5p could bind to SATB1. Besides, SATB1 was significantly overexpressed in cancer tissues of patients with lung adenocarcinoma, and SATB1 overexpression accelerated the migration and proliferation of A549/H1650 cells and reversed inhibition on cell migration and proliferation by miR‐191‐5p.ConclusionOverexpression of miR‐191‐5p is capable of blocking the migration and proliferation of lung cancer cells, and its mechanism may be through targeting SATB1 thus downregulating Wnt signaling.
Highlights
Adenocarcinoma, lung adenocarcinoma-induced mortality rate is still high (Diaz-Garcia et al, 2013; Rosell, Bivona, & Karachaliou, 2013), which may be associated with early metastasis (Peters et al, 2012)
Our study revealed miR-191-5p's therapeutic effects on inhibiting cancer cell migration and proliferation and further identified its target gene SATB homeobox 1 (SATB1) (HGNC: 10541) and downstream pathway
According to Pearson correlation analysis, miR-191-5p had a negative relationship to SATB1 expression in lung cancer tissues (r2 = 0.352, p = .0022) (Figure 4b)
Summary
Lung cancer is the leading cause of cancer-related deaths worldwide, and there are approximately 1.4 million new cases of lung cancer every year (Jemal et al, 2011) (Wei, Tian, Song, Wu, & Liu, 2018). To investigate the function of miR-191-5p in lung adenocarcinoma and its possible mechanism. The effects of miR-191-5p and SATB1 on cell proliferation and migration were examined through the CCK-8 and Transwell assays. The binding relationships between miR-191-5p and SATB1 were confirmed by dual-luciferase reporter gene assay. Overexpression of miR-191-5p repressed the migration and proliferation of lung cancer A549/H1650 cells. According to the reporter gene assay, miR-191-5p could bind to SATB1. SATB1 was significantly overexpressed in cancer tissues of patients with lung adenocarcinoma, and SATB1 overexpression accelerated the migration and proliferation of A549/H1650 cells and reversed inhibition on cell migration and proliferation by miR-191-5p. Conclusion: Overexpression of miR-191-5p is capable of blocking the migration and proliferation of lung cancer cells, and its mechanism may be through targeting SATB1 downregulating Wnt signaling
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
