Abstract
Lung cancer as one of the commonest invasive malignancies is featured by high morbidity and mortality, wherein lung adenocarcinoma (LUAD) is the most prevalent subtype. Accumulating evidence exhibited that microRNAs are involved in LUAD occurrence and progression. In this study, miR-182-5p was observed to increase in both LUAD tissue and cell lines. Overexpression of miR-182-5p could prominently facilitate cell proliferation, migration, and invasion in LUAD. Through bioinformatics analysis, STARD13 was theorized as the target gene of miR-182-5p, which was lowly expressed in LUAD. Further molecular experiments manifested that miR-182-5p bound to the 3′-untranslated region of STARD13, and there was an inverse correlation between STARD13 and miR-182-5p in LUAD. Rescue experiments demonstrated that silencing STARD13 conspicuously restored the inhibitory effect of decreased miR-182-5p on cell proliferation, migration, and invasion in LUAD. Together, our findings revealed novel roles of the miR-182-5p/STARD13 axis in LUAD progression.
Highlights
Lung adenocarcinoma (LUAD) as the most prevalent histological subtype of lung cancer is responsible for 40% of all lung cancer cases
Li et al [6] found that miR-182-5p is upregulated in oral squamous cell carcinoma (OSCC) cells and clinical samples, as well as functions as an oncogene in OSCC via hindering CAMK2N1 expression. miR-182-5p is identified as an oncogene in ovarian cancer [7], breast cancer and [8], melanoma [9], whereas it exerts an inhibitory role in renal cell carcinoma [10, 11] and bladder cancer [12]
Total proteins were isolated through radioimmunoprecipitation assay (RIPA) (Beyotime, Shanghai, China), and the concentration of which was measured by bicinchoninic acid (BCA) protein assay kit (Pierce Biotechnology, Rockford, IL, USA)
Summary
Lung adenocarcinoma (LUAD) as the most prevalent histological subtype of lung cancer is responsible for 40% of all lung cancer cases. MiR-182-5p as a neotype cancer-related miRNA is extensively reported to play a regulatory role in various kinds of tumors. In non-small-cell lung cancer (NSCLC), miR-182 acts an oncogenic role and fosters cell proliferation by directly targeting FBXW7 and FBXW11, indicating that miR-182 may be a fresh diagnostic and prognostic biomarker. STARD13 is downregulated in prostate cancer, and its overexpression hinders proliferation of cancer cells [15]. It is considered a tumor repressor in hepatocellular carcinoma [16]. This study deeply investigated the expression and molecular mechanism of miR-182-5p and STARD13 in LUAD, and our results may lay a theoretical basis for the discovery of therapeutic targets underlying LUAD
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
