MiR-16-5p modulates the radiosensitivity of cervical cancer cells via regulating coactivator-associated arginine methyltransferase 1.

Abstract

This study was to investigate the expression of coactivator-associated arginine methyltransferase 1 (CARM1) and miR-16-5p in cervical cancer (CC), and explore their roles in radioresistance. Western blot and immunohistochemistry were used to detect the expression of CARM1 in tissues and cells. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of miR-16-5p. CC cells received different doses of X-ray exposure, and then cell counting kit-8 method and colony formation assay were used to detect cell proliferation. Apoptosis was detected by flow cytometry. Then we used Targetscan database to predict that CARM1 is a potential target of miR-16-5p, and further verified the targeting relationship between them by western blot, RT-PCR and dual luciferase reporter experiments. We demonstrated that CARM1 were highly expressed in CC tissues and radio-resistant CC cells, while miR-16-5p expression was low. Under irradiation, up-regulation of CARM1 can induce radiotherapy resistance of CC cells, while overexpression of miR-16-5p or CARM1 knockdown could inhibit the survival of CC cell and induced apoptosis. CARM1 was verified as a target for miR-16-5p. Besides, up-regulation of CARM1 reversed the increase in radiosensitivity induced by miR-16-5p. Collectively, we concluded that miR-16-5p promoted the radiosensitivity of CC cells by targeting CARM1.