Abstract
Background: Breast cancer endangers the life of women and has become the major cause of deaths among them. MiRNAs are found to exert a regulatory effect on the migration, proliferation and apoptosis of breast cancer cells. This research aims at investigating the miR-16-5p expression and its effect on the pathogenesis of breast cancer. Methods: Their clinical data were analyzed with qRT-PCR. CCK8, EdU and Transwell was performed to explore the function of miR-16-5p in cell migration and proliferation of breast cancer cells. Dual-luciferase reporter assay, immunohistochemistry and Western blotting were carried out to explore the relation between miR-16-5p and AKT3. Results: It was discovered that miR-16-5p was lowly expressed in breast cancer patients. Meanwhile, breast cancer patients with under-expressed miR-16-5p had a lower survival rate than those with highly expressed miR-16-5p. Furthermore, decreased miR-16-5p in cell and animal models enhanced migration and proliferation of breast cancer cells, stimulated cell cycle and reduced cell apoptosis. Finally, we found miR-16-5p restrained the NF-κB pathway and decreased AKT3 gene, thereby suppressing the breast cancer development. Conclusion: It can be seen that miR-16-5p exhibits a low expression in breast cancer tissues, which can inhibit breast cancer by restraining the NF-κB pathway and elevating reducing AKT3.
Highlights
As the most common tumor, breast cancer is still an issue to be resolved in the world [1]
We found that miR-16-5p in breast cancer cells lines was prominently lower than that in MCF 10A cell line, in which the highest was in MCF-7 cell line and the lowest in BT-549 cell line (Figure 1B)
Increasing show that miRNAs significantly contribute to the maintenance of normal cell growth and function, and changes in miRNAs expression has a close relationship to cancer [25,26,27]
Summary
As the most common tumor, breast cancer is still an issue to be resolved in the world [1]. According to the global cancer statistics, there were more than 1.7 million new cases of breast cancer in 2012, taking up 25% of the incidence rate of all cancers [2]. In spite of these depressed statistics, advanced diagnosis methods, introduced molecular gene signature platforms and breast cancer management contribute to more effective treatment and regulation strategies, reducing the mortality rate of breast cancer [3]. Decreased miR-16-5p in cell and animal models enhanced migration and proliferation of breast cancer cells, stimulated cell cycle and reduced cell apoptosis. We found miR-16-5p restrained the NF-κB pathway and decreased AKT3 gene, thereby suppressing the breast cancer development. Conclusion: It can be seen that miR-16-5p exhibits a low expression in breast cancer tissues, which can inhibit breast cancer by restraining the NF-κB pathway and elevating reducing AKT3
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