MiR-155 Regulates the Proliferation of Glioma Cells Through PI3K/AKT Signaling.

Changzhen Wang,Dahao Wu

doi:10.3389/fneur.2020.00297

Changzhen Wang, Dahao Wu

Open Access

https://doi.org/10.3389/fneur.2020.00297

Copy DOI

Abstract

Objective: Micro-RNA plays a critical role in the pathological process of gliomas. Previous research showed that the level of miR-155 was significantly increased in many cancers, including gliomas. However, the mechanism of glioma is still unknown.Method: To investigate the regulatory function of miR-155 on glioma U87-MG cells and its effects on related signaling pathways. After transfection of miR-155 mimic and inhibitor, the level of miR-155 were applied to detect cell proliferation, apoptosis, senescence index, invasive ability and cell migration at different time points (0, 24, 24 h, respectively) by CCK8 assay, flow cytometry, β-galactosidase (β-gal) staining, transwell and scratch test, respectively. The effect of miR-155 on PI3K/AKT signal pathway was observed at meantime.Results: Compared with the control group, after miR-155 mimic transfection, U87-MG cell viability, cell migration rate and invasiveness were increased, while apoptosis and senescence were significantly decreased, which was the opposite on miR-155 inhibitor transfection. The phosphorylation levels of miR-155, PI3K, AKT, PI3K, and AKT in U87-MG cells intervened with miR-155 mimic also increased significantly, while the levels of PTEN, Caspase-3, Caspase-9 mRNA, and protein declined significantly, with statistically significant difference. Meanwhile, compared with the control group, miR-155 inhibitor group were on the contrary.Conclusion: The study indicated that miR-155 take charge a key function in regulating the proliferation, migration, and invasion of glioma U87-MG cells through PI3K/AKT signaling pathway, and has anti-glioma effects by inhibition of miR-155, which provided ideas for further clinical treatment of glioma patients.

Highlights

Glioma is the primary malignant brain tumor of the central nervous system, which often occurs in children, adolescent, and middle-aged population [1]
Domestic and overseas studies have confirmed that miR-21 can affect the growth and invasion and other biological behaviors of leukemic K562 cells, lung cancer cells and gastric cancer cells through PTEN/PI3K/AKT signaling pathway [13,14,15]
The results showed that NC had no significant effect on apoptosis, while miR-155 mimic group could significantly inhibit the early and late apoptosis of U87MG cells, with the total number of apoptotic cells decreased, whereas miR-155 inhibitor group could significantly promote the early and late apoptosis of U87-MG cells (P < 0.05) (Figures 3A–E)

Summary

Introduction

Glioma is the primary malignant brain tumor of the central nervous system, which often occurs in children, adolescent, and middle-aged population [1]. Domestic and overseas studies have confirmed that miR-21 can affect the growth and invasion and other biological behaviors of leukemic K562 cells, lung cancer cells and gastric cancer cells through PTEN/PI3K/AKT signaling pathway [13,14,15]. It has been demonstrated that miR-34a mediates cisplatin-induced cell death via regulating PI3K/AKT/survivin pathway in gastric cancer [16], while miR20a can induce radiation resistance effect by activating hepatoma PTEN/PI3K/Akt signal path [17]. These multi-miRNA can exert biological functions in tumor diseases through PI3K/AKT. The research will study this theory, so as to provide new ideas to treat the glioma

Methods

Results

Conclusion