Abstract
BackgroundIt has been shown that miR-144-3p regulates cell proliferation, apoptosis, migration and invasion in various cancers. However, the function and expression of miR-144-3p in colorectal cancer (CRC) remained obscure.MethodsImmunohistochemical (IHC) staining was performed to investigate the protein expression of BCL6 in CRC tissues. The effect of BCL6 and miR-144-3p on CRC cells was explored through methylthiazolyl tetrazolium (MTT) assay, colony formation and cell cycle assays. Luciferase reporter assays, reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot assay were carried out to determine that BCL6 is directly regulated by miR-144-3p.ResultsOur results showed that miR-144-3p is down-regulated in CRC and correlates with the tumor progression of CRC patients. miR-144-3p inhibits cell proliferation and delays G1/S phase transition of CRC cells. Moreover, we found that BCL6 is a new target of miR-144-3p. Furthermore, BCL6 is a mediator of miR-144-3p repression of cell proliferation and cell cycle arrest in CRC cells. miR-144-3p repression of Wnt/β-catenin signaling is mediated by BCL6 in CRC cells.ConclusionsOverall, the effect of the miR-144-3p/BCL6 axis on regulating CRC carcinogenesis was demonstrated, and miR-144-3p was identified as a potential prognostic and therapeutic target in colorectal cancer.
Highlights
It has been shown that miR-144-3p regulates cell proliferation, apoptosis, migration and invasion in various cancers
B-cell lymphoma 6 (BCL6) is a mediator of miR-144-3p repression of cell proliferation and cell cycle arrest in colorectal cancer (CRC) cells. miR-144-3p repression of Wnt/β-catenin signaling is mediated by BCL6 in CRC cells
Expression of miR-144-3p is down-regulated in CRC tissues and related to the clinicopathologic characteristics of patients To explore the potential role of miR-144-3p in the progression of CRC, we examined the level of miR-144-3p in 20 CRC tissues and non-tumor adjacent tissues using qRTPCR
Summary
It has been shown that miR-144-3p regulates cell proliferation, apoptosis, migration and invasion in various cancers. The function and expression of miR-144-3p in colorectal cancer (CRC) remained obscure. Recent reports show that abnormal expression of miR-144-3p plays either tumor suppressor or oncogene roles in various cancers. In hepatocellular carcinoma (HCC), miR-144-3p acts as a tumor suppressor microRNA to regulate the progression of HCC [9, 10]. MiR-144-3p inhibits cell proliferation, migration, and invasion through targeting AP-1 transcription factor subunit (FOSB) and promotes cell apoptosis and cell cycle arrest by targeting proline-rich protein 11 [11, 12]. MiR-144-3p presents an oncogene role in clear cell renal cell carcinoma (ccRCC) by regulating ARID1A [13]. The function of miR-144-3p in CRC remained unknown
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
