Abstract
The aim of the present study was to explore the mechanism through which miR-130a-3p affects the viability, proliferation, migration, and invasion of nasopharyngeal carcinoma (NPC). Tissue samples were collected from the hospital department. NPC cell lines were purchased to conduct the in vitro and in vivo assays. A series of biological assays including MTT, Transwell, and wound healing assays were conducted to investigate the effects of miR-130a-3p and BACH2 on NPC cells. MiR-130a-3p was down-regulated in both NPC tissues and cell lines, whereas BACH2 was up-regulated in both tissues and cell lines. MiR-130a-3p overexpression inhibited NPC cell viability, proliferation, migration, and invasion but promoted cell apoptosis. The converse was true of BACH2, the down-regulation of which could inhibit the corresponding cell abilities and promote apoptosis of NPC cells. The target relationship between miR-130a-3p and BACH2 was confirmed. The epithelial–mesenchymal transition (EMT) pathway was also influenced by miR-130a-3p down-regulation. In conclusion, miR-130a-3p could bind to BACH2, inhibit NPC cell abilities, and promote cell apoptosis.
Highlights
Nasopharyngeal carcinoma (NPC) is a malignant tumor that occurs with high frequency in Southeast Asia and Northern Africa [1]
This was consistent in comparison with expression levels of the NP69 cell line, where the expression of miR-130a-3p was significantly lower in these NPC cell lines (i.e. CNE, CNE-2Z, S18, HONE-1, and C666-1)
MiRNAs have been reported to play an important role in NPC and its prognosis [26,27]
Summary
Nasopharyngeal carcinoma (NPC) is a malignant tumor that occurs with high frequency in Southeast Asia and Northern Africa [1]. In 2008, there were an estimated 84400 cases of NPC and 51600 deaths [2]. The major etiological factors of NPC are genetic susceptibility, environmental conditions and Epstein–Barr virus (EBV) infection [1]. It has been reported that certain foods that were high in nitroso compounds and volatile nitrosamines can increase the risk of NPC. The most common treatment for NPC is radiation therapy and the 5-year survival rate of stage I and stage II patients treated with radiotherapy has been up to 90% [3]. A recent report showed that the percentage of NPC patients with regional lymph node metastasis and distant metastasis were 74.5 and 19.9%, respectively [5]. It is necessary to investigate the molecular bases of NPC metastasis in order to discover new therapeutic approaches
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