Abstract
BackgroundDysregulated miRNAs are involved in carcinogenesis of the breast and may be used as prognostic biomarkers and therapeutic targets during the cancer process. The purpose of this study was to explore the effect of miR-105-3p on the tumourigenicity of breast cancer and its underlying molecular mechanisms.MethodsReverse transcription-quantitative polymerase chain reaction (RT-qPCR) was applied to detect the expression of miR-105-3p in breast cancer tissues and cell lines. The impacts of miR-105-3p on the proliferation, migration, invasion and apoptosis of human breast cancer cells (MCF-7 and ZR-75-30) were evaluated by CCK-8 assays, Transwell chamber assays, TUNEL assays and western blot analyses. In addition, bioinformatics and luciferase reporter assays were used to determine the target genes of miR-105-3p.ResultsThe expression of miR-105-3p was elevated in breast cancer tissues and increased with tumour severity. Downregulation of miR-105-3p could inhibit cell proliferation, suppress cell migration/invasion, and promote cell apoptosis in MCF-7 and ZR-75-30 cells. Furthermore, Golgi integral membrane protein 4 (GOLIM4) was identified as the direct target gene of miR-105-3p by bioinformatics and luciferase reporter assays. In addition, silencing GOLIM4 restored the anti-breast cancer effects induced by miR-105-3p downregulation.ConclusionsMiR-105-3p acts as an oncogene to promote the proliferation and metastasis of breast cancer cells by targeting GOLIM4, which provides a new target for the prevention and treatment of breast cancer.
Highlights
Dysregulated miRNAs are involved in carcinogenesis of the breast and may be used as prognostic biomarkers and therapeutic targets during the cancer process
The expression levels of miR-105-3p in breast cancer tissues and cell lines The expression of miR-105-3p in breast cancer and adjacent noncancerous tissues of 80 breast cancer patients with different stages was detected with Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
To choose suitable breast cancer cell lines to evaluate the biological function of miR-105-3p, we determined the expression levels of miR-105-3p in several breast cancer cell lines, including MCF10A, MDA-MB-231, SKBr-3, MCF-7 and ZR75-30, by RT-qPCR
Summary
Dysregulated miRNAs are involved in carcinogenesis of the breast and may be used as prognostic biomarkers and therapeutic targets during the cancer process. Bahena et al found that overexpression of miR-10b in breast cancer cells could inhibit PTEN expression, promote epithelial-mesenchymal transition (EMT), and upregulate stem cell markers expression, thereby promoting breast cancer invasion and metastasis [10]. Damiano and his colleagues found that the downregulation of miR-200 in breast cancer could account for EMT and stem-like features of breast cancer by targeting ZEB1 [11]. All these studies revealed the important roles of miRNAs in breast cancer
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