MiR-744-3p regulates keratinocyte proliferation and differentiation via targeting KLLN in psoriasis.

Abstract

Psoriasis is a chronic inflammatory disease, and microRNAs have been reported to regulate the pathogenesis of psoriasis. Up-regulated miR-744-3p level was identified to associate with psoriasis while the precise functions of miR-744-3p in psoriasis were not well-elucidated. We first confirmed the up-regulation of miR-744-3p in psoriasis by measuring its expression level in psoriatic samples. We explored the roles of miR-744-3p on keratinocytes proliferation and differentiation. We searched the targets of miR-744-3p and evaluated the roles of one target, KLLN on keratinocytes proliferation and differentiation. We confirmed the up-regulation of miR-744-3p in psoriatic samples. MiR-744-3p promoted keratinocytes proliferation while inhibited differentiation. MiR-744-3p targeted KLLN and overexpression of miR-744-3p resulted in decreased expression of KLLN. Overexpression of KLLN prevented the effects of miR-744-3p on keratinocytes proliferation and differentiation. MiR-744-3p regulated the proliferation and differentiation of keratinocytes through targeting KLLN in psoriasis.