Abstract
Introduction. The biological roles of microRNA-654-5p (miR-654-5p) in cancers have been previously reported. However, its role in colorectal cancer (CRC) remains largely unknown. The purpose of this work was to investigate the roles and associated mechanisms in CRC. Methods Quantitative Real-Time PCR (qRT-PCR) was utilized to explore the expression pattern of miR-654-5p in CRC cells. Cell Counting Kit-8 (CCK-8) assay, wound-healing assay, and transwell invasion assay were conducted to investigate the effects of miR-654-5p on CRC cell proliferation, migration, and invasion, respectively. Moreover, the mechanisms behind miR-654-5p regulates CRC progression were investigated. Results Compared with normal cell line, miR-654-5p expression level was significantly suppressed in CRC cells. After overexpression of miR-654-5p, the malignancy behaviors of CRC cells including cell proliferation, migration, and invasion were remarkably decreased. Subsequently, we found hematopoietic cell-specific protein 1-associated protein X-1 (HAX-1) was a putative target for miR-654-5p. Rescue experiments showed overexpression of HAX-1 could partially reversed the effects of miR-654-4p on CRC cell events. Conclusion miR-654-5p was reduced expression in CRC cells and could regulate CRC progression via targeting HAX-1.
Highlights
About 1.2 million newly diagnosed colorectal cancer (CRC) patients and resulted in 551 thousand deaths of in 2018 at the worldwide range [1]. e incidence of CRC in Asian countries has rapidly increased in past decades [2]
Alterations in lifestyle and eating habits were considered as two major reasons to explain this phenomenon [2]. e pathogenesis of CRC is accompanied with the abnormal expression of multiple tumor suppressor genes and oncogenes [3]. erefore, a deep investigation of the abnormal expressed genes is useful to understand mechanisms behind CRC development
Bioinformatical Analysis. e target of miR-654-5p was analyzed at TargetScan V_7.2. Among all these predicted targets, hematopoietic cellspecific protein 1-associated protein X-1 (HAX-1) was selected for further analyses as it was reported to be overexpressed in CRC tissues [10]
Summary
About 1.2 million newly diagnosed colorectal cancer (CRC) patients and resulted in 551 thousand deaths of in 2018 at the worldwide range [1]. e incidence of CRC in Asian countries has rapidly increased in past decades [2]. Tan and co-authors showed low expression of miR654-5p in breast cancer was closely associated with advanced tumor stages and poorer overall survival [8]. They showed miR-654-5p functions a tumor suppressive role to regulate breast cancer cell growth and invasion via targeting epithelial stromal interaction 1 [8]. Lu and co-authors showed miR-654-5p was able to promote oral squamous cell carcinoma cell growth, metastasis, and chemoresistance through the targeting Grb-2-related adaptor protein/Ras/MAPK signaling pathway, indicating an oncogenic role of miR-6545p [9]. Our purpose was to explore the biological functions of miR-654-5p in CRC and to deeply investigate the potential mechanisms involved in the miR-654-5pmediated effects on CRC cells
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