Abstract
Background: MiR-654-3p can repress malignant progression of cancer cells, whereas no relative reports were about its modulatory mechanism in sinonasal squamous cell carcinoma (SNSCC). This research committed to approaching modulatory effect of miR-654-3p on SNSCC cells. Methods: Bioinformatics methods were utilized for analyzing interaction of miR-654-3p/cAMP-responsive element binding protein 1 (CREB1)/presenilin-1 (PSEN1). Expression levels of miR-654-3p, CREB1, and PSEN1 mRNA were assessed by quantitative real-time polymerase chain reaction. Western blot was completed for level assessment of CREB1, PSEN1, and epithelial–mesenchymal transition–related proteins. The targeted relationship between miR-654-3p and CREB1, or CREB1 and PSEN1 was authenticated via dual-luciferase assay and ChIP assay. A trail of experiments in vitro was used for detection of the effects of miR-654-3p/CREB1/PSEN1 axis on malignant progression of SNSCC cells. Results: CREB1 as the downstream target mRNA of miR-654-3p could activate transcription of its downstream target gene PSEN1. Besides, miR-654-3p could target CREB1 to repress PSEN1 expression, thus restraining proliferation, migration, invasion, epithelial–mesenchymal transition, and hastening apoptosis of SNSCC cells. Conclusion: MiR-654-3p as an antitumor gene targeted CREB1 to hamper malignant progression of SNSCC through miR-654-3p/CREB1/PSEN1 axis.
Highlights
Sinonasal squamous cell carcinoma (SNSCC) is a malignancy derived from nasal sinuses, making up 65% of all cases of rhinocarcinoma (Al-Qurayshi et al, 2020)
Analyses from perspectives of etiology, epidemiology, clinical features, and relatively genetic profiles yield out that SNSCC have markedly different features from other head and neck squamous cell carcinoma, and SNSCC can be regarded as a solitary carcinoma (Llorente et al, 2014)
To confirm the influence of miR-654-3p on proliferative potential of SNSCC cells, CCK-8 assay was conducted on each transfection group
Summary
Sinonasal squamous cell carcinoma (SNSCC) is a malignancy derived from nasal sinuses, making up 65% of all cases of rhinocarcinoma (Al-Qurayshi et al, 2020). Analyses from perspectives of etiology, epidemiology, clinical features, and relatively genetic profiles yield out that SNSCC have markedly different features from other head and neck squamous cell carcinoma (cancer of pharynx, MiR-654-3p Hinders SNSCC Malignant Progression oral carcinoma), and SNSCC can be regarded as a solitary carcinoma (Llorente et al, 2014). MiR-654-3p can repress malignant progression of cancer cells, whereas no relative reports were about its modulatory mechanism in sinonasal squamous cell carcinoma (SNSCC). This research committed to approaching modulatory effect of miR-654-3p on SNSCC cells
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