Abstract
Breast cancer is the most common female cancer in the world. Breast cancer patients are currently treated with a combination of surgery, chemotherapy, radiotherapy, and targeted therapy, but the 5-year overall survival rate is still low. Therefore, we plan to explore the potential interaction mechanism between miR-622 and EYA1 in the breast cancer cells and their effect on proliferation, migration, and invasion of breast cancer, to lay a foundation for the gene therapy of breast cancer and improve the therapeutic effect. This study found that miR-622 was highly expressed in breast cancer cell lines, while EYA1 was poorly. In MCF-7 cell line, miR-622 had the highest expression level, while EYA1 had the lowest. Besides, the bioinformatics analysis showed that EYA1 possesses putative miR-622 binding sites within its 3 ′ UTR. The increased miR-622 significantly enhanced the proliferation, migration, and invasion of MCF-7 cell line and inhibited luciferase reporter activity in the 3 ′ UTR of EYA1-WT. When upregulating the expression of miR-622, the mRNA and protein expression levels of EYA1 were significantly decreased. We also found that the silencing of EYA1 promoted the proliferation, migration, and invasion of breast cancer MCF-7 cell line. These results indicate that miRNA-622 plays a tumor-promoting role in breast cancer through targeted negative regulation of EYA1, suggesting that miRNA-622 may become a potential target for breast cancer treatment.
Highlights
Breast cancer is a malignant tumor growing in the epithelial tissue of the breast
We found that the overexpression of miR-622 significantly promoted cell proliferation, migration, and invasion compared with the mimic or its negative control (miR-NC) or control group (Figures 2(b)–2(f)), which indicates that miR-622 might serve as a tumor promoter in regulating breast cancer biological process
In metastatic breast cancer, the expression levels of miR-21 and miR-105 were significantly higher than those in healthy subjects [25, 26]. miR-21 was significantly associated with the advanced clinical stage, the metastasis of the cancer cells in a lymph node, and poor prognosis [27]
Summary
Breast cancer is a malignant tumor growing in the epithelial tissue of the breast. In recent years, the incidence of breast cancer among women in the world has been rising continuously, surpassing lung cancer for the first time and becoming the largest cancer in the world [1, 2]. It is estimated that by the end of 2020, there will be about 279,100 new confirmed cases of breast cancer in the United States alone [3]. It is the second leading cause of cancer-related deaths among women in the world [3, 4]. It is of great significance to further study the molecular mechanism and signal transduction pathway of breast cancer progression and find new targets for improving breast cancer treatment methods and improving patients’ quality of life
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