MiR‐605‐5p promotes invasion and proliferation by targeting TNFAIP3 in non–small‐cell lung cancer

Abstract

Lung cancer is an significant cause of death worldwide, and non-small-cell lung cancer (NSCLC) is the most common type of lung cancer. MicroRNAs (miRNAs) have been identified to play key roles in NSCLC development. Recently, it has beenreported that miR-605-5p is a cancer-related miRNA in several types of tumors. In this study, we study the role of miR-605-5p in NSCLC cells. We find that miR-605-5p is upregulated in NSCLC cells. Overexpression of miR-605-5p significantly promotes lung cancer invasion and migration in H460 and H1299 cells. Besides this, miR-605-5p also promotes lung cancer cell carcinoma proliferation and metastasis in vivo. However, downregulation of miR-605-5p inhibitscell invasion and migration byinhibiting lung cancer cell carcinoma proliferation and metastasis. In addition,the luciferase report assay identifies 3'-untranslated region tumor necrosis factor α-induced protein 3 (TNFAIP3) asa target of miR-605-5p. Silencing of TNFAIP3 promotesinvasion and proliferation in lung cancer. In addition, the knockdown of TNFAIP3 restores the significant decrease in invasion and proliferation in miR-605-5p-inhibitor-transfected lung cancer cells. In conclusion, miR-605-5p promotes invasion and proliferation by targeting TNFAIP3 in NSCLC, andmay provide possible biomarkers for NSCLC therapy.