MiR-599 targeting TOP2A inhibits the malignancy of bladder cancer cells

Abstract

BackgroundRecent research suggests that many miRNAs influence the development of bladder cancer (BC). However, the function of the miR-599/TOP2A axis in BC cells is still unclear. Our aim was to verify the effect of the miR-599/TOP2A axis on BC progression. MethodsThe expression of TOP2A and miR-599 in BC tissues and cells was detected using RT-qPCR. Luciferase assays, RIP assays, and RNA pull-down assays were performed to determine the interaction between miR-599 and TOP2A. CCK-8, flow cytometry-based apoptosis, wound healing, and Transwell assays were utilized to determine the proliferation, migration, invasion and apoptosis of BC cells. ResultsMiR-599 was significantly downregulated in the BC, and miR-599 overexpression impeded the malignancy of BC cells by regulating proliferation, migration, invasion and apoptosis. TOP2A proved to a downstream target of miR-599 could enhance the tumorigenesis phenotype of BC cells. The experimental results also indicated that miR-599 reversed the oncogenic effects induced by TOP2A. ConclusionOur study revealed that miR-599 represses BC tumorigenesis by inhibiting TOP2A.