MiR-543 promotes tumorigenesis and angiogenesis in non-small cell lung cancer via modulating metastasis associated protein 1

Dawei Wang,Wenjun Li,Xudong Tian,Li Cai

doi:10.1186/s10020-020-00175-1

Abstract

ObjectiveThis study is aimed to explore the role of miR-543 in non-small cell lung cancer (NSCLC), and verify whether miR-543 targets metastasis associated protein 1 (MTA1) to affect tumorigenesis and angiogenesis in NSCLC.MethodsFirstly, miR-543 mimic and inhibitor were transfected into A549 cells and H1299 cells. The cells proliferation was tested by MTT and clone formation. The cells apoptosis was analyzed by cytometry. Tube formation assay was used to measure the vascularization of cells. qRT-PCR and Western Blot were used to measure the MTA1 expression. Dual-luciferase assay was used to analyze whether miR-543 targets MTA1. Secondly, MTA1 mimic and inhibitor were transfected into cells to analyze the effect of MTA1 on proliferation and angiogenesis in NSCLC cells. Lastly, the nude mice were used to verify the effect of miR-543 on tumorigenesis and angiogeneisis in NSCLC via modulating MATA1.ResultsmiR-543 overexpression could apparently promote cells proliferation and angiogeneisis in NSCLC cells. Meanwhile, the MTA1 expression was increased after transfecting miR-543 mimic. Dual luciferase reporter assay revealed MTA1 was a downstream target of miR-543. Further studies showed that inhibition of MTA1 weakened the role of miR-543 overexpression in NSCLC cells. Vivo experiments revealed that miR-543 promoted cells proliferation and angiogenesis in tumor tissues via modulating MTA1.ConclusionmiR-543 could target MTA1 to promote tumorigenesis and angiogenesis in NSCLC via targeting MTA1.

Highlights

As the leading cause of cancer deaths, the incidence rate and mortality rate of lung cancer are increasing, which poses a great threat to people’s health and life (Siegel et al 2017)
The cells activities of A549 were obviously raised in miR-543 mimic group (p < 0.05), while the cell activities of A549 were markedly reduced in miR-543 siRNA group (p < 0.05)
We found that the relative lumen number was notably raised in miR-543 mimic group, but decreased in miR-543 overexpression up-regulated the expression of metastasis associated protein 1 (MTA1) in A549 and H1299 cells The effect of miR-543 on MTA1 expression in A549 and H1299 cells was researched by qRT-PCR and western blot, respectively (Fig. 3)

Summary

Introduction

As the leading cause of cancer deaths, the incidence rate and mortality rate of lung cancer are increasing, which poses a great threat to people’s health and life (Siegel et al 2017). About 80–85% of lung cancers are MicroRNAs (miRNAs) are a class of non-coding RNA molecules encoded by endogenous genes with a length of about 22 nucleotides (Guiot et al 2019). MiRNAs have been revealed to play a crucial role in the tumorigenesis of lung cancer. Research on miR-543 has recently become an interested item. Wang et al Molecular Medicine (2020) 26:44 be abnormally expressed in a number of tumors, such as gastric cancer, oral squamous cell carcinoma, etc. Overexpression or knockdown miRNAs would cause the corresponding changes in the phenotype of cancer cells. The effect of miR543 in NSCLC has not been accurately verified

Methods

Results

Discussion

Conclusion