Abstract
Purpose: To explore the effects of miR-541-3P on the expression of heat shock transcription factor 1 (HSF1) in gastric cancer cells (GC).Methods: The MicroRNA Target Prediction Database was used to predict whether miR-541-3p interacts with HSF1. Interaction was assessed by dual-luciferase reporter assays. Furthermore, miR-541-3p mRNA levels in GC cell lines were determined by qRT-PCR. Human GC cell lines MKN45 and NCI-N87 were transfected with miR-541-3p mimic. Cell apoptosis, proliferation, invasion, and migration were evaluated using flow cytometry, apoptosis assays, Edu assays, CCK-8 assays, and transwell assays, respectively. Caspase-3, Bcl-2, and cleaved caspase-3 expression levels were determined by western blot.Results: Expression of miR-541-3p was significantly down-regulated in GC cells. Functionally, miR-541-3p mimic inhibited GC cell proliferation, migration, and invasion and induced apoptosis in vitro (p <0.01). Mechanistically, miR-541-3p interacted with HSF1 and inhibited its expression. Overexpression of HSF1 counteracted the effects of miR-541-3p mimic in GC cells.Conclusion: These results indicate that miR-541-3p suppresses the development of GC by targeting HSF1 and thus, is a possible strategy for for the management of GC.
Highlights
Gastric cancer (GC) is a digestive tract disease with high incidence that is often life threatening [1]
Expression of miR-541-3p mRNA was significantly lower in GC cells than GES1 cells (Figure 1 A), and the MKN45 and NCI-N87 cell lines were selected for subsequent experiments
These findings suggest that downregulation of miR-541-3p represses GC cell proliferation
Summary
Gastric cancer (GC) is a digestive tract disease with high incidence that is often life threatening [1]. Due to a high mortality rate and frequent diagnosis at an advanced stage of disease, GC is the third most common cause of cancer deaths [2]. Gastric cancer is associated with various risk factors, such as Helicobacter pylori infection, cigarette smoking, and alcohol consumption [4]. The early stages of GC are often asymptomatic, and if symptoms are present at the time of diagnosis, the disease is often advanced and incurable [3]. It is important to find new treatments for GC and investigate the mechanisms of the disease
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