Abstract
Background: Modifications of miRNA expression have been related to various types of cancers including hepatocellular carcinoma (HCC). miRNAs directly act as repressors of gene expression, as they reside in fragile sites, as well as cancer-related genomic regions. Notch signaling is a conserved evolutionary pathway that controls cell functions. The dysregulation of this pathway leads to different diseases such as cancer. Objectives: This study aimed to investigate the role of miR-515-5p and Notch1 as new diagnostic markers in HCC. Methods: Forty formalin fixed paraffin embedded (FFPE) autopsy blocks and 40 FFPE normal liver tissues were selected from the archives of the pathology of Imam Reza hospital, Tabriz, Iran. Real-time polymerase chain reaction (PCR) was used for gene expression. Immune histochemistry method was used for detecting notch1 in normal and cancer FFPE tissues. Hematoxylin and eosin staining was also used for the diagnosis of normal and cancerous tissues. Results: miR-515-5P showed higher expression in the cancer group compared to the normal group (4.7 fold). Hematoxylin and eosin staining of HCC tissues showed significant color intensity than that of normal tissues. Immune histochemistry results revealed significant Ag-Ab reaction in the cancer group. In this study, we analyzed miRNA gene expression and notch 1 level in HCC patients. miRNA dysregulation has been found in a large variety of HCCs. Hepatocarcinogenesis was associated with the expression level of miR-515-5p with carcinogenesis. Moreover, notch1 was a key protein in liver cell fate and a progressive molecule in HCC. Conclusion: Our study demonstrated the main role of miR-515-5p in the pathogenesis of HCC. Likewise, it disclosed the expression of these genes could be utilized in HCC prognosis.
Highlights
Modifications of miRNA expression have been related to various types of cancers including hepatocellular carcinoma (HCC). miRNAs directly act as repressors of gene expression, as they reside in fragile sites, as well as cancer-related genomic regions
For a long-term storage, human tissue samples are fixed in formalin and inserted in paraffin for decades [6]
Many institutes hold a vast number of books on paraffin-block that make it possible to preserve neoplasm samples such as those of sporadic tumors for a long time [7]
Summary
Modifications of miRNA expression have been related to various types of cancers including hepatocellular carcinoma (HCC). miRNAs directly act as repressors of gene expression, as they reside in fragile sites, as well as cancer-related genomic regions. Modifications of miRNA expression have been related to various types of cancers including hepatocellular carcinoma (HCC). Notch signaling is a conserved evolutionary pathway that controls cell functions The dysregulation of this pathway leads to different diseases such as cancer. We analyzed miRNA gene expression and notch 1 level in HCC patients. Conclusion: Our study demonstrated the main role of miR-515-5p in the pathogenesis of HCC Likewise, it disclosed the expression of these genes could be utilized in HCC prognosis. MicroRNAs (miRNAs) control essential functions especially gene expression and have a major effect on post-transcriptional regulation of gene expression [8]. Alterations in microRNA expression have been associated with various types of Hepatocellular biomolcules cancers These miRNAs act as gene suppressors and are located at fragile sites and cancer-associated genomic regions. Various pathological features viz human malignancies, circulatory, and metabolic diseases have been connected with miRNA dysregulation [12]
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