MiR-506 Affects Cervical Cancer Cell Resistance to Adriamycin (ADM) Through Regulating Signal Transducer and Activator of Transcription 3 (STAT3)

Abstract

STAT3 is involved in the development and resistance of several tumors. Abnormal miR-506 expression is associated with cervical cancer. miR-506 was predicted to have a relationship with STAT3. Our study investigated miR-506's role in cervical cancer cell adriamycin (ADM) resistance. The ADM drug-resistant cell line, Caski/ADM was established followed by measuring miR-506 and STAT3 mRNA level by qRT-PCR and STAT3 protein expression by Western blot. The Caski/ADM cells were separated into miR-NC group and miR-506 mimic group followed by analysis of STAT3 and p-STAT3 level by Western blot, cell apoptosis and proliferation by flow cytometry. There was a targeted regulatory relationship between miR-506 and STAT3 mRNA. miR-506 in Caski/ADM cells was significantly downregulated compared to that in Caski cells, which further significantly reduced compared to HCerEpiC cells. Caski/ADM cells had highest STAT3 level followed by Caski cells and HCerEpiC cells. miR-506 mimic transfection significantly upregulated miR-506, decreased STAT3 and p-STAT3 level with increased cell apoptosis and decreased cell proliferation as well as resistance. miR-506 level regulates cervical cancer ADM resistance. Increasing miR-506 expression down-regulates STAT3 expression, inhibits proliferation of cervical cancer cells, promotes apoptosis and reduces ADM resistance.