Abstract
Background Colorectal cancer is the third frequent tumor in the whole world. MiR-483, located at the 11p15.5 locus, acts as an oncogene in multiple tumors. The purpose of this study is to explore the important roles of miR-483 in colorectal cancer. Materials and Methods RT-qPCR and western blot were applied to calculate the mRNA levels of miR-483 and genes. The Kaplan–Meier method was conducted to calculate the survival of patients with colorectal cancer. The proliferation and invasive abilities were measured by Methyl Thiazolyl Tetrazolium (MTT) and transwell assays. Results MiR-483 was upregulated in colorectal cancer tissues, and the upregulation of miR-483 predicted poor prognosis of colorectal cancer patients. NDRG2 was a target gene of miR-483 in colorectal cancer. Furthermore, miR-483 has been reported to promote colorectal cancer cell proliferation and invasion through targeting NDRG2 by the PI3K/AKT pathway and epithelial-to-mesenchymal transition (EMT). In addition, the overexpression of miR-483 promoted xenograft growth of LOVO cells. Conclusion MiR-483 promoted cell proliferation through the NDRG2/PI3K/AKT pathway and invasion-mediated EMT in colorectal cancer. In view of the multiple mechanisms of molecular immunotherapy, it is necessary to further study the relationship between miR-483 and colorectal cancer, so as to find a more direct and effective treatment method to prevent colorectal cancer.
Highlights
Colorectal cancer (CRC) is a type of gastrointestinal cancer that is the third frequent tumor in the whole world [1, 2]
Upregulation of MiR-483 Predicted Poor Prognosis of Colorectal Cancer. e mRNA level of miR-483 was calculated in 47 pairs of colorectal cancer and nonnecrotic colon tissues
The expression of miR-483 was higher in colorectal cancer tissues in comparison with corresponding nonnecrotic colon tissues (P < 0.05) (Figure 1(a))
Summary
Colorectal cancer (CRC) is a type of gastrointestinal cancer that is the third frequent tumor in the whole world [1, 2]. A previous study has reported several miRNAs played important roles in colorectal cancer, including miR-483 [8]. MiR-483, which has been reported to be located at the 11p15.5 locus, plays important roles in multiple tumors, including breast cancer, nasopharyngeal carcinoma, and anaplastic thyroid cancer [9,10,11]. MiR-483 has been reported to promote colorectal cancer cell proliferation and invasion through targeting NDRG2 by the PI3K/AKTpathway and epithelial-to-mesenchymal transition (EMT). MiR-483 promoted cell proliferation through the NDRG2/PI3K/AKT pathway and invasion-mediated EMT in colorectal cancer. In view of the multiple mechanisms of molecular immunotherapy, it is necessary to further study the relationship between miR483 and colorectal cancer, so as to find a more direct and effective treatment method to prevent colorectal cancer
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