Abstract
BackgroundmiRNAs have been reported to be involved in multiple biological processes of gliomas. Here, we aimed to analyze miR-4310 and its correlation genes involved in the progression of human glioma.MethodsmiR-4310 expression levels were examined in glioma and non-tumor brain (NB) tissues. The molecular mechanisms of miR-4310 expression and its effects on cell proliferation, migration, and invasion were explored using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide, Transwell chamber, Boyden chamber, and western blot analyses, as well as its effect on tumorigenesis was explored in vivo in nude mice. The relationships between miR-4310, SP1, phosphatase, and tensin homolog (PTEN) were explored using chromatin immunoprecipitation, agarose gel electrophoresis, electrophoresis mobility shift, and dual-luciferase reporter gene assays.ResultsmiR-4310 expression was upregulated in glioma tissues compared to that in NB tissues. Overexpressed miR-4310 promoted glioma cell proliferation, migration, and invasion in vitro, as well as tumorigenesis in vivo. The inhibition of miR-4310 expression was sufficient to reverse these results. Mechanistic analyses revealed that miR-4310 promoted glioma progression through the PI3K/AKT pathway by targeting PTEN. Additionally, SP1 induced the expression of miR-4310 by binding to its promoter region.ConclusionmiR-4310 promotes the progression of glioma by targeting PTEN and activating the PI3K/AKT pathway; meanwhile, the expression of miR-4310 was induced by SP1.
Highlights
MiRNAs have been reported to be involved in multiple biological processes of gliomas
Results miR‐4310 promotes glioma cell proliferation, migration, and invasion in vitro To explore the roles of miR-4310 in glioma progression, we first compared the expression of miR-4310 between 8 non-tumor brain (NB) tissues and 26 glioma tissues
We found that glioma tissues showed higher levels of miR-4310 than NB tissues (Fig. 1a)
Summary
MiRNAs have been reported to be involved in multiple biological processes of gliomas. We aimed to analyze miR-4310 and its correlation genes involved in the progression of human glioma. Glioma is the most common primary intraparenchymal central nervous system (CNS) tumor. In the revised 2016 WHO classification of CNS tumors, numerous molecular. PTEN is a common tumor suppressor gene. The function of PTEN mainly involves the regulation of the PI3K/ AKT pathway. PTEN antagonizes the function of PI3K by dephosphorylating PIP3 to PIP2, thereby regulating the effect of the AKT downstream pathway. PTEN negatively regulates the PI3K/AKT pathway to mediate cell reproduction, invasion, and migration [6,7,8]. Studies have shown that PTEN is frequently mutated in various cancers, including gliomas.
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