MiR-429-3p/LPIN1 Axis Promotes Chicken Abdominal Fat Deposition via PPARγ Pathway.

Xiaohuan Chao,Xiquan Zhang,Lijin Guo,Shudai Lin,Kang Luan,Qingbin Luo,Qi Wang,Manqing Liu,Qinghua Nie,Jinqi Jiang,Wen Luo,Weiling Huang

doi:10.3389/fcell.2020.595637

Abstract

To explore the regulatory mechanism of abdominal fat deposition in broilers, 100-day-old Sanhuang chickens (n = 12) were divided into high-fat and low-fat groups, according to the abdominal fat ratio size. Total RNA isolated from the 12 abdominal fat tissues was used for miRNA and mRNA sequencing. Results of miRNA and mRNA sequencing revealed that miR-429-3p was highly expressed in high-fat chicken whereas LPIN1 expression was downregulated. Further, we determined that miR-429-3p promoted preadipocyte proliferation and differentiation, whereas LPIN1 exerted an opposite effect. Notably, we found that the miR-429-3p/LPIN1 axis facilitated PPARγ pathway activation, which is closely associated with the progression of adipogenesis. In conclusion, our results provide evidence that a novel miR-429-3p/LPIN1 axis is involved in the regulation of adipogenesis, which may have a guiding role in the improvement of breeding for abdominal fat traits in broiler chickens.

Highlights

Obesity is becoming a world-wide health problem, which is believed to increase the risk of psychological problems, fatty liver disease, cardiovascular disease, and even cancer (Bray, 2004; Swinburn et al, 2011; Hruby and Hu, 2015; Lauby-Secretan et al, 2016; Bugianesi, 2020)
The clean data was mapped to chicken GRCg6a genome and gene set analysis showed that a total of 18,165 genes were detected. | Fold change | ≥ 2 and q-value ≤ 0.001 were considered as the cut-off for differentially expressed genes (DEGs), and we obtained 1,562 DEGs from mRNA sequencing
MiR-429-3p/LPIN1 axis may be an important regulator of adipogenesis, and we focused on this axis in follow-up experiments

Summary

Introduction

Obesity is becoming a world-wide health problem, which is believed to increase the risk of psychological problems, fatty liver disease, cardiovascular disease, and even cancer (Bray, 2004; Swinburn et al, 2011; Hruby and Hu, 2015; Lauby-Secretan et al, 2016; Bugianesi, 2020). A number of obesity-associated genes have been identified in adipogenesis regulation, such as melanocortin-4 receptor (MC4R) (Kuhnen et al, 2019), uncoupling protein-1 (UCP1) (Fedorenko et al, 2012), and pro-opiomelanocortin (POMC) (Qiu et al, 2014). Investigation into obesity utilizing a chicken model can gain new insights into the mechanism underlying fat deposition and provide a possible solution for the public obesity problem

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