Abstract
Objective: Multiple gene targets have been reported for treatment of non-small cell lung cancer (NSCLC), however, the accompanying genetic tolerance was reported increasingly. Therefore, it is important to find new biomarkers or therapeutic targets in treatment of NSCLC.Methods: The expression levels of miR-371b-5p were detected by qRT-PCR in NSCLC tissues and cell lines. To evaluate the effect of miR-371b-5p on NSCLC progression, we first transfected the miR-371b-5p inhibitor for construction of the miR-371b-5p down-regulated cell model. Then the cell proliferation, migration, invasion and cell apoptosis were detected. In addition, the expression levels of adhesion factors were detected. The target gene of miR-371b-5p was identified by bioinformatics analysis, and rescue experiment was conducted to validate the effect of miR-371b-5p on proliferation, migration and invasion of NSCLC.Results: Our findings revealed that the miR-371b-5p was overexpressed in NSCLC and could markedly promote the cell proliferation, migration and invasion. Expression levels of both intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were significantly down-regulated when treated by miR-371b-5p inhibitor. Moreover, dual-luciferase reporter assay showed that the miR-371b-5p targeted SCAI in regulation of cell proliferation, migration and invasion, and the expression of miR-371b-5p was negatively associated with SCAI in NSCLC tissues and cell lines. Rescue experiment revealed that the miR-371b-5p could rescue the effect of SCAI on cell proliferation, migration and invasion.Conclusion: Our results suggest that the miR-371b-5p and SCAI may serve as novel prognostic biomarkers and therapeutic targets for NSCLC.
Highlights
Lung cancer is reported to be one of the leading causes of deaths in both males and females worldwide [1]
We evaluate the expression levels of miR-371b-5p in human non-small cell lung cancer (NSCLC), and discovered that the miR-371b-5p was aberrantly up-regulated in both human NSCLC tissues and NSCLC cell lines
We found that the silencing of this miRNA could significantly inhibited cell proliferation and viability, migration and invasion in H1299 and H466 cell lines via directly targeting suppressor of cancer cell invasion (SCAI)
Summary
Lung cancer is reported to be one of the leading causes of deaths in both males and females worldwide [1]. The non-small cell lung cancer (NSCLC) represents approximately 85% of all lung cancer types [2]. Despite developments in the clinical oncology technology, the prognosis of NSCLC is poor with only 15% of 5-year survival rate [3]. Increasing studies have proved that the progression of NSCLC is closely associated with a variety of gene abnormalities [4]. The identification of diver oncogenic alterations as well as the involved key pathways is of great importance in understanding the lung cancer pathogenesis and development. Recent study illustrates that several gene targets have been reported for treatment of NSCLC, the accompanying genetic tolerance are reported increasingly [5]
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