MiR-370-3p Regulates Adipogenesis through Targeting Mknk1.

Peiwen Zhang,Yang Gu,Qian Xiao,Linyuan Shen,Guoqing Tang,Yanzhi Jiang,Shuang Wu,Xutao Lin,Shunhua Zhang,Xinrong Li,Lei Chen,Lili Niu,Ye Zhao,Xinyu Guo,Li Zhu

doi:10.3390/molecules26226926

Peiwen Zhang, Yang Gu + Show 13 more

Open Access

https://doi.org/10.3390/molecules26226926

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Abstract

Excessive fat accumulation can lead to obesity, diabetes, hyperlipidemia, atherosclerosis, and other diseases. MicroRNAs are a class of microRNAs that regulate gene expression and are highly conserved in function among species. microRNAs have been shown to act as regulatory factors to inhibit fat accumulation in the body. We found that miR-370-3p was expressed at lower levels in the fat mass of mice on a high-fat diet than in mice on a normal control diet. Furthermore, our data showed that the overexpression of miR-370-3p significantly suppressed the mRNA expression levels of adipogenic markers. Thus, miR-370-3p overexpression reduced lipid accumulation. Conversely, the inhibition of miR-370-3p suppressed 3T3-L1 preadipocyte proliferation and promoted preadipocyte differentiation. In addition, Mknk1, a target gene of miR-370-3p, plays an opposing role in preadipocyte proliferation and differentiation. Moreover, consistent results from in vitro as well as in vivo experiments suggest that the inhibition of fat accumulation by miR-370-3p may result from the inhibition of saturated fatty acids that promote the accumulation of polyunsaturated fatty acids. In conclusion, these results suggest that miR-370-3p plays an important role in adipogenesis and fatty acid metabolism through the regulation of Mknk1.

Highlights

Obesity is a major global public health problem [1] and is closely linked with hypertension, cardiovascular disease [2], type 2 diabetes, etc
White adipose tissue is an endocrine organ involved in the functioning of endocrine, autocrine and paracrine signaling. miR-370-3p is implicated in many critical biological processes such as cancer cell migration and invasion
The expression of miR-370-3p was found to be downregulated in the adipose tissues of high-fat diet (HFD)-fed mice and gradually decreased during 3T3-L1 preadipocyte differentiation

Summary

Introduction

Obesity is a major global public health problem [1] and is closely linked with hypertension, cardiovascular disease [2], type 2 diabetes, etc. Obesity results from energy intake exceeding expenditure [3]. The adipose development process involves preadipocyte proliferation and adipocytes differentiation [4]. Adipogenesis is a crucial process that participates in adipocyte development and fat metabolism [5,6]. It is necessary to determine the adipogenesis mechanisms that are closely linked with severe diseases. A high number of studies have demonstrated that adipogenesis is tightly regulated by transcriptional and posttranscriptional regulation factors, and complex signaling pathways

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Conclusion