Abstract
Laryngeal carcinoma is a malignant disease with high morbidity and mortality. Several studies have indicated that miRNA dysfunction involves in the development of laryngeal carcinoma. In this study, the connection of miR-339-5p and laryngeal carcinoma was investigated, and qRT-PCR, CCK-8, and flow cytometry assay were used to observe the function of miR-339-5p on laryngeal carcinoma. Besides, the target database, dual-luciferase reporter assay, and western blot were used to explore the regulation mechanism of miR-339-5p on the progression of laryngeal carcinoma. The results showed that miR-339-5p was significantly downregulated in cisplatin-resistant cells of laryngeal carcinoma, and miR-339-5p upregulation could weaken the resistance of laryngeal carcinoma cells on cisplatin. Moreover, miR-339-5p could directly react with 3′-UTR of TAK1, and TAK1 could reverse the effects of miR-339-5p on the progression of autophagy. In conclusion, this study suggests that miR-339-5p can inhibit the autophagy to decrease the cisplatin resistance of laryngeal carcinoma via targeting TAK1.
Highlights
Laryngeal carcinoma is the second most prevalent malignancy of the upper aerodigestive tract, which is associated with several factors such as tobacco and alcohol consumption [1, 2]
We found that miR-339-5p played an important role in decreasing the resistance of laryngeal carcinoma cells on cisplatin. miR-339-5p has been confirmed as a tumor inhibitor to suppress the formation and development of the tumors
The results in this study showed that TAK1 upregulation could reverse the effects of miR-339-5p on depressing the cisplatin resistance of laryngeal carcinoma cells
Summary
Laryngeal carcinoma is the second most prevalent malignancy of the upper aerodigestive tract, which is associated with several factors such as tobacco and alcohol consumption [1, 2]. The overall 5-year survival rate of the patients with laryngeal carcinoma is only 64.2% [3]. MicroRNAs (miRNAs), a class of noncoding RNA with short chain, are responsible for transcriptional inhibition of mRNAs to regulate the expression of the related proteins in various eukaryotic cells [9, 10]. These small RNAs have been accepted as important molecular regulators in cellular life activities by many researchers [11, 12]. Many studies have demonstrated that the dysfunction of miRNAs can facilitate tumor growth, invasion, migration, and drug resistance [13]. We investigate the connection of miR-3395p and laryngeal carcinoma and aimed to provide some reference for laryngeal carcinoma treatment
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