Abstract
MicroRNAs, considered as a promising focus for the treatment of tumors, are key regulators of a large number of genes. The aim of the present study was to investigate the biological functions of microRNA (miR)-330-3p in liver cancer as it had been identified previously that miR-330-3p was deregulated in liver cancer. In order to identify the function of miR-330-3p in liver cancer, the expression of miR-330-3p was determined in liver cancer tissues and adjacent non-tumor tissues using reverse transcription-quantitative polymerase chain reaction analysis. To elucidate the function of miR-330-3p in liver cancer, miR-330-3p was overexpressed using mimic transfection. Cell migration was inhibited by miR-330-3p in liver cancer cells. The miRNA target prediction databases were used to identify potential target genes of miR-330-3p in liver cancer. The RNA level of mitogen-activated protein kinase kinase 1 (MAP2K1) was downregulated by miR-330-3p in liver cancer cells. In conclusion, miR-330-3p suppresses cell migration by targeting MAP2K1 in liver cancer cells.
Highlights
Mature microRNAs are a group of short non‐coding RNAs containing between 18 and 26 nucleotides, which, at the post‐transcriptional level, regulate target gene expression by targeting the 5' or 3'‐untranslated regions (UTRs) of mRNA [4]. miRNAs regulate a number of biological processes, and their dysregulation has been demonstrated to be associated with development, proliferation, stress resistance, metastasis and apoptosis of cancer cells by regulating oncogenes or tumor suppressors [5], including in liver cancer
Investigation into miRNAs has led to novel studies to identify the molecular mechanism of cancer cell metastasis [13,14], and the development of potential therapeutics in the treatment of human cancer
Previous results support a fundamental function of microRNAs in tumor invasion and metastasis; these biological functions are associated with the deregulation of microRNAs in various types of cancer [14]
Summary
Mature microRNAs (miRNAs) are a group of short non‐coding RNAs containing between 18 and 26 nucleotides, which, at the post‐transcriptional level, regulate target gene expression by targeting the 5' or 3'‐untranslated regions (UTRs) of mRNA [4]. miRNAs regulate a number of biological processes, and their dysregulation has been demonstrated to be associated with development, proliferation, stress resistance, metastasis and apoptosis of cancer cells by regulating oncogenes or tumor suppressors [5], including in liver cancer. MiRNAs regulate a number of biological processes, and their dysregulation has been demonstrated to be associated with development, proliferation, stress resistance, metastasis and apoptosis of cancer cells by regulating oncogenes or tumor suppressors [5], including in liver cancer. MiR‐330‐3p has been identified to be deregulated in malignant liver cancer [9]. The association of miR‐330‐3p with liver cancer and its function in liver cancer remains unclear. The most effective treatment for liver cancer is currently surgery [2]. The characteristics of liver cancer, including multifocal development or distant metastasis, preclude surgical treatment for the patients from being curative [3]. In order to determine the biology of liver cancer, it is important to gain a thorough understanding of the underlying molecular mechanisms of tumor growth and metastasis
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