MiR-30d is related to asbestos exposure and inhibits migration and invasion in NCI-H2452 cells.

Li Ju,Min Yu,Wei Wu,Junqiang Chen,Jianlin Lou,Yun Xiao,Lijin Zhu,Wei Li,Tianhui Chen,Xing Zhang,Zhaoqiang Jiang,Zhenyu Jia,Xianhong Yin,Shibo Ying

doi:10.1002/2211-5463.12274

Abstract

Pleural malignant mesothelioma (MM) is a highly aggressive tumor that is typically related to asbestos exposure and has a latency of 20–60 years. Several microRNA contribute to MM initiation and progression, but the mechanisms are not clear. Here, we found that miR‐30d is downregulated in the pleural MM cell line NCI‐H2452, in the plasma of asbestos‐exposed individuals, and in asbestos‐exposed mesothelial cells. Furthermore, we investigated the influence of the overexpression of miR‐30d in pleural MM cells. We demonstrated that miR‐30d overexpression could suppress pleural MM cell proliferation, migration, and invasion in vitro and could promote cell apoptosis but could not significantly influence cell cycle. The mRNA and protein expression of vimentin and TWIST1 decreased, and the mRNA expression of CDH1 increased in NCI‐H2452 cells that overexpressed miR‐30d. We therefore conclude that miR‐30d is related to asbestos exposure and inhibits cell migration and invasion by regulating the epithelial–mesenchymal transition in NCI‐H2452 cells.

Highlights

Li Ju1, Wei Wu1, Xianhong Yin1,2, Yun Xiao1, Zhenyu Jia1, Jianlin Lou1, Min Yu1, Shibo Ying1, Tianhui Chen1, Zhaoqiang Jiang1, Wei Li3, Junqiang Chen1, Xing Zhang1 and Lijin Zhu1
Asbestos-exposed subjects were household handspinning women workers and workers in asbestos factories; nonasbestos exposure participants included individuals who were not exposed to asbestos who had family members who were not exposed to asbestos
We found that miR-30d was downregulated in the plasma of asbestos-exposed subjects

Summary

Introduction

Li Ju1, Wei Wu1, Xianhong Yin, Yun Xiao, Zhenyu Jia, Jianlin Lou, Min Yu1, Shibo Ying, Tianhui Chen, Zhaoqiang Jiang, Wei Li3, Junqiang Chen, Xing Zhang and Lijin Zhu. Pleural malignant mesothelioma (MM) is a highly aggressive tumor that is typically related to asbestos exposure and has a latency of 20– 60 years. More recent evidence has indicated that a class of small noncoding RNA referred to as microRNA (miRNA) contribute to the initiation and progression of a number of tumors, including pleural MM [4].

Methods

Results

Discussion

Conclusion