Abstract
This study aimed to investigate the role and relevant mechanism of miR-30a-3p action in asthma. The results of this study revealed that the expression levels of miR-30a-3p were significantly decreased in the peripheral blood of asthmatic patients. In addition, we found that the CC chemokine receptor (CCR3) was a target of miR-30a-3p. Subsequently, an asthma mouse model was established using ovalbumin (OVA). The results showed that the expression of miR-30a-3p and CCR3 was downregulated and upregulated, respectively, in the peripheral blood of asthmatic mice. Enzyme-linked immunosorbent assay (ELISA) in asthmatic mouse serum demonstrated that miR-30a-3p mimic treatment significantly decreased the secretion of OVA-specific IgE, eotaxin-1, interleukin (IL)-5, and IL-4. These results suggested that miR-30a-3p inhibited CCR3 signaling pathway and relieved the inflammatory response against asthma in vivo. Eosinophils have also been implicated in the asthmatic inflammatory response. Therefore, the in vitro effects of miR-30a-3p on eosinophil activity were determined. Findings suggested that miR-30a-3p mimic significantly reduced eosinophil viability and migration and induced apoptosis. In addition, CCR3 and eotaxin-1 downregulation were observed. The aforementioned results were significantly reversed following CCR3 overexpression. This study suggested that miR-30a-3p was involved in asthma by regulating eosinophil activity and targeting CCR3.
Highlights
Asthma is a very common chronic inflammatory disease and a leading cause of morbidity in children and adults worldwide [1,2]
The results revealed that miR-30a-3p expression was significantly downregulated in asthmatic patients compared with that noted to healthy volunteers (Figure 1)
Eosinophils were co-transfected for 48 h with chemokine receptor 3 (CCR3)-WT, CCR3-MUT, miR-30a-3p mimic, or negative control
Summary
Asthma is a very common chronic inflammatory disease and a leading cause of morbidity in children and adults worldwide [1,2]. The main clinical features of asthma are wheezing, shortness of breath, chest tightness, and cough. The incidence of asthma in different countries ranges and affects 1–18% of the general population, whereas it is estimated that approximately 300 million people worldwide suffer from this disease [4]. The increasing incidence and mortality of asthma that has been noted in recent years are possibly attributed to its complex pathogenesis. Bousquet et al demonstrated that eosinophilia was associated with the severity of asthma [5]. Blood eosinophil counts provide a readily available multifunctional biomarker for severe eosinophilic asthma [6,7]
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