MiR-26b inhibits the prostate cancer cell's migration and invasion

Abstract

Objective To investigate the effects of the up-regulated expression of microRNA (miRNA,miR)-26b on the biological properties of prostate cancer cells.Methods The expression of miR-26b in PC23,PC-3M,DU-145,LNCaP,22RV1 cell lines and benign prostate tissue were detected by Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR).miR-26b mimics and control miRNA were transfected into LNCaP cells by Lipofectamine 2000.The cellular growth activity was assayed by methyl thiazol tetrazolium (MTT) assay,the apoptosis and cell cycles was tested by flow cytometry.Wound healing assay and Transwell assay were applied to measure cell migration and invasion.Results The expression of miR-26b in prostate cancer cell lines was lower than that in benign prostate tissue.Compared to the control,the expression of miR-26b was increased by 310 folds in LNCaP transfected with miR 26b,which numbers of migrated and penetrated reduce significantly from (158 ± 16) and (147 ± 15) to (29 ±3) and (30 ±2) respectively (P ＜0.05),whereas,cellular growth activity,apoptosis and cell cycles were not changed significantly.Conclusion The expression of miR-26b is down-regulation in prostate cancer cell lines.Up-regulation of miR-26b inhibits LNCaP cell' s migration and invasion. Key words: Prostate cancer; microRNA-26b; Cell migration; Cell invasion