MiR-2467 is a Potential Marker for Prediction of Gestational Diabetes Mellitus in Pregnancy.

Abstract

The current study aims to detect the serum level of miR-2467 in pregnant women with gestational diabetes mellitus (GDM) and analyze its clinical significance. The study included 67 pregnant women with GDM and 60 pregnant women with normal glucose tolerance as control group. The serum miR-2467 level of pregnant women was detected by RT-PCR. The diagnostic efficiency of serum miR-2467 for GDM was analyzed using Receiver Operating Characteristic (ROC) curve, and the risk factors of GDM were analyzed by logistic regression analysis. Pearson's correlation assay was used to analyze the correlation between serum miR-2467 and clinical indicators. The possible target gene of miR-2467 was predicted using TargetScan and validated using dual luciferase reporter assay. The body mass index (BMI), TC, TG, LDL-C, FPG, HbA1c, HOMA-IR, and serum miR-2467 levels in the GDM group were higher than those in the control group. The serum miR-2467 level of GDM pregnant women was positively correlated with the levels of TC, TG, LDL-C, FPG, HbA1c, and HOMA-IR. The AUC of miR-2467 was 0.876 for GDM pregnant women. Logistic analysis showed that serum miR-2467 level was an independent risk factor for GDM. A conserved binding site was identified in the 3'UTR of adiponectin, and dual luciferase reporter assay showed that adiponectin was a target gene of miR-2467. Altogether, the high level of serum miR-2467 can be used for the preliminary screening of GDM. Targeting the regulation of miR-2467/adiponectin might be a new strategy for the prevention of GDM.