MiR‑24 may be a negative regulator of menin in lung cancer.

Yunhu Pan,Fangfang Huang,Yangyang Liu,Hongmei Wang,Fangyu Cao,Debin Ma,Zhiyu Ji,Yitan Chen,Yushu Dong,Limin Luo

doi:10.3892/or.2018.6327

Abstract

The incidence of lung cancer in China increases annually, and effective targets for the diagnosis and treatment of lung cancer are urgently needed. miRNAs are currently considered to be involved in the regulation of tumor development and growth. miR‑24has been found to contribute to the development of several tumors. Menin is a key tumor suppressor gene, and its expression is generally low in lung cancer. The effects of miR‑24 on the biological behavior of lung cancer cells were detected by MTT and Transwell assays. In the present study, miR‑24 was found to be associated with menin, affecting the activity of the SMAD3 pathway in lung cancer by inhibiting menin expression. miR‑24 may promote the growth and metastasis and inhibit the apoptosis of lung cancer cells by targeting menin. Therefore, the aim of the present study was to provide a new theoretical basis for the targeted therapy of lung cancer.

Highlights

According to WHO statistics, the incidence of lung cancer in China increases annually by ~25% [1,2]
The results revealed that the expression of miR‐24 in the tumor tissues was found to be higher compared with that in the adjacent tissues of 70 lung cancer patients as determined by qPCR (Fig. 1A)
In hepatocellular carcinoma (HCC), miR‐24 functions as an oncogene, at least in part by promoting cell invasion through downregulation of p53 [24], and miR‐24‐3p plays an important role in the initiation and progression of HCC by targeting metallothionein 1M [25]

Summary

Introduction

According to WHO statistics, the incidence of lung cancer in China increases annually by ~25% [1,2]. Mutations of key proto‐oncogenes, such as K‐ras, ALK and EGFR, and tumor‐suppressor genes, such as p53, are important molecular mechanisms underlying the occurrence of lung cancer [3,4,5,6,7]. MiRNAs have been attracting increasing attention in the research of tumor pathogenesis, [8,9,10] and their sensitivity and specificity for the diagnosis of lung cancer have been found to be relatively high [11,12]. A critical tumor‐suppressor gene in multiple endocrine neoplasia type 1, encodes a recently identified protein, menin, of which the emerging roles in cancer development have been attracting increasing attention [13]. The molecular mechanisms underlying the role of menin in lung cancer remain unclear

Objectives

Methods

Results

Conclusion