Abstract
BackgroundTo investigate the effect of miR-221-5p on cell proliferaton and metastasis of human prostate cancer in vitro and vivo.MethodsWe established PC3 cell lines with stable overexpression or silencing of miRNA-221-5p via lentivirus infection. miRNA-221-5p and its target gene SOCS1 expression levels in the stable cells were analyzed by real-time polymerase chain reaction (RT-PCR) and western blotting. Using luciferase reporter assays to study the relationship between miR-221-5p and SOCS1. Cell proliferative activity was measured using the MTT assay and colony formation assay. Migration ability was assessed using wound-healing assay and transwell assay. To further study the function of miR-221-5p in human prostate cancer we established nude mice xenograft model in vivo.ResultsmiR-221-5p regulates the proliferation, migration of prostate cancer cells in vitro and tumorigenesis in vivo by regulating socs1 expression through targeted its 3’UTR, and miR-221-5p regulates MAPK/ERK signaling pathway and EMT features in prostate cancer cells.ConclusionsUp-regulation and silencing of miR-221-5p expression in prostate cancer cells are correlated with cell proliferation, migration and tumorigenesis, which suggest that miR-221-5p plays an important role in prostate cancer progression.
Highlights
To investigate the effect of miR-221-5p on cell proliferaton and metastasis of human prostate cancer in vitro and vivo
We investigated that miR-221-5p accelerates cell growth, migration and tumor development of human prostate cancer cells in vitro and vivo, and miR-221-5p regulates Mitogen-activated protein kinase (MAPK)/Extracellular signal regulated kinase (ERK) signaling pathway and Epithelial-mesenchymal transition (EMT) features in prostate cancer cells
We have found that the expression of miR-221-5p is significantly different between tumor tissues and adjacent tissues of prostate cancer patients (Fig. 1a). the functions of these miRNAs in the progression of prostate cancer remained unexplored
Summary
To investigate the effect of miR-221-5p on cell proliferaton and metastasis of human prostate cancer in vitro and vivo. Shao et al BMC Urology (2018) 18:14 transcriptional level to be master regulators of many important biological processes, such as cell growth, invasion, metastasis, and apoptosis, etc. We investigated the potential functions of miR-221-5p in prostate cancer and found that miR-221-5p can specific target SOCS1 (Suppressers of cytokine signaling (SOCS) family protein, which is tumor suppressor genes [22,23,24,25]. We investigated that miR-221-5p accelerates cell growth, migration and tumor development of human prostate cancer cells in vitro and vivo, and miR-221-5p regulates MAPK/ERK signaling pathway and EMT features in prostate cancer cells
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