MiR-21 is up-regulated in urinary exosomes of chronic kidney disease patients and after glomerular injury.

Tim Lange,Nadine Artelt,Panagiotis Kavvadas,Uwe Lendeckel,Karlhans Endlich,Sylvia Stracke,Frances Kindt,Rainer Rettig,Nicole Endlich,Christos Chatziantoniou,Christos E Chadjichristos

doi:10.1111/jcmm.14317

Abstract

With 500 million people affected worldwide, chronic kidney disease (CKD) constitutes a major public health problem.1 Mostly arising from arterial hypertension and diabetes mellitus, it is a terminal disease without any causal therapy, leading to dialysis and/or kidney transplantation. Glomerulopathies are the main cause of end stage renal disease (ESRD), which are mainly caused by podocyte effacement or loss.2 A relevance of microRNAs (miRs) on podocyte function and the pathogenesis of glomerulopathies3 could have been clarified. Both, their molecular influence on translation and their potential role as biomarkers for kidney diseases, including CKD, became of major research interest. Especially miRs derived from urinary exosomes appear to have a promising potential as biomarkers, since they can be accessed non‐invasively and they are protected against degradation,4 making sample preparation more unsusceptible against environmental influences. Unfortunately, only little is known about exosomal miR expression in urine of CKD patients. Therefore, the present study aims at the investigation of the expression levels of the well‐investigated miRs miR‐21, miR‐30a‐5p and miR‐92a in urinary exosomes of CKD patients, representing a new promising, non‐invasive sample type and their functional roles in different injury models.

Highlights

With 500 million people affected worldwide, chronic kidney disease (CKD) constitutes a major public health problem.[1]
MiRs derived from urinary exosomes appear to have a promising potential as biomarkers, since they can be accessed non‐invasively and they are protected against degra‐ dation,[4] making sample preparation more unsusceptible against environmental influences
We used a randomly picked, widespread set of patients suffering from CKD

Summary

| INTRODUCTION

With 500 million people affected worldwide, chronic kidney disease (CKD) constitutes a major public health problem.[1]. A relevance of microRNAs (miRs) on podocyte function and the pathogenesis of glomerulopathies[3] could have been clarified. Both, their molecular influence on translation and their potential role as biomarkers for kidney diseases, including CKD, became of major research interest. MiRs derived from urinary exosomes appear to have a promising potential as biomarkers, since they can be accessed non‐invasively and they are protected against degra‐ dation,[4] making sample preparation more unsusceptible against environmental influences. The present study aims at the investigation of the expression levels of the well‐investigated miRs miR‐21, miR‐30a‐5p and miR‐92a in urinary exosomes of CKD patients, representing a new promising, non‐invasive sample type and their functional roles in different injury models

| MATERIAL AND METHODS

| DISCUSSION

Findings

CONFLICT OF INTEREST