Abstract
This study assesses miR-21’s role in non-small cell lung cancer (NSCLC) cells. miR-21/PTEN RNA level in normal and NSCLC cell (A549) was detected by qPCR. After transfection of miR-21 and inhibitors, cell proliferation was assessed by MTT. PTEN, NF-κB, p53, BCL2, BAX levels were measured after si-PTEN transfection. miR-21 was significantly lower and PTEN was higher in NSCLC cells than normal cells (P <0.001) and PTEN was negatively associated with miR-21. MTT assay showed that cell survival rate was decreased after miR-21 was inhibited and increased after miR-21 was overexpressed. After inhibiting PTEN, protein expression of proliferation-related factors NF-κB and p53 was decreased. TUNEL experiment showed elevated cell apoptosis after miR-21 was inhibited and decreased apoptosis after miR-21 was overexpressed. Inhibition of PTEN modulated Akt signaling as demonstrated by no changes of Akt expression and decreased p-Akt level along with downregulated BCL2 and BAX. In conclusion, miR-21/PTEN inhibits NSCLC cell proliferation and promotes cell apoptosis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
