Abstract
microRNAs (miRNAs) play an important role in gene regulation in animals by pairing with target gene mRNA. Many miRNAs are differentially expressed in the adipose tissue, often with conserved expression. In our study, we found that miR-208b expression was observed differently in the preadipocyte differentiation model. When miR-208b was overexpressed in the preadipocyte differentiation model, the overexpressed group displayed higher expression of PPARγ and FABP4—the markers of preadipocyte differentiation. Oil Red O staining revealed that the count of lipid droplets was increased in the overexpressed group. When the expression of miR-208b was inhibited, the above indicators showed an opposite trend. Moreover, results from both 5-ethynyl-2′-deoxyuridine (EDU) and cell counting kit (CCK) analysis showed that miR-208b promoted the proliferation of preadipocyte. Expression of gene CSNK2A2, a direct miR-208b target, was downregulated in the overexpressed group, providing a possible link to multiple signal pathways. Overall, our data indicate that miR-208b play a positive regulatory effect on the proliferation and differentiation of rabbit preadipocyte.
Highlights
During the past five decades, the prevalence of obesity has rapidly increased, becoming a serious public health issue [1]
To establish a model of rabbit preadipocyte differentiation, the third generation of preadipocyte was cultured in cell culture plates and differentiated with the aforementioned method when the density reached 80%
MiR-208b positively promoted the expression of preadipocyte differentiation key genes PPARγ and FABP4 at mRNA and protein levels
Summary
During the past five decades, the prevalence of obesity has rapidly increased, becoming a serious public health issue [1]. Obesity is characterized by the excess accumulation of adipose tissue under the definition of the World Health Organization (WHO) [2]. Proliferation and differentiation of preadipocyte induce the number of mature adipocyte capable of triglyceride storage, metabolism, and production of adipokines [4]. Rabbit is an ideal experimental animal to study adipogenesis due to its lipid metabolism and obesity-related clinical manifestations similar to those of humans [5,6]. A detailed study of the regulatory mechanisms that underlie rabbit preadipocyte proliferation and differentiation would be expected to contribute to the understanding of adipogenesis and rabbit breeding
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