Abstract
Epithelial–mesenchymal transition (EMT) plays a pivotal role in cancer progression. Hsa-miR-205 is considered one of the fundamental regulators of EMT. In the present study, we found that miR-205 was down-regulated in glioma tissues and human glioma cells U87 and U251. Meanwhile, miR-205 overexpression enhanced E-cadherin, reduced mesenchymal markers, and decreased cell proliferation, migration, and invasion in vitro. In vivo, miR-205 suppressed tumor growth. Additionally, HOXD9 was confirmed as a direct target of miR-205. Suppression of HOXD9 by miR-205 was demonstrated by luciferase reporter assay, quantitative real time-PCR analysis, and western blot. Moreover, we observed a negative correlation between miR-205 and HOXD9 in human glioma tissues. In summary, our findings demonstrated that miR-205 suppresses glioma tumor growth, invasion, and reverses EMT through down-regulating its target HOXD9.
Highlights
Gliomas are the most commonly diagnosed primary tumors of the brain [1]
Response to therapy is dependent on multiple factors, including tumor growth rate and invasiveness, and there is an urgent need for therapies directed against newly discovered molecular targets, those involved in epithelial–mesenchymal transition (EMT)
We identified HOXD9 as a target of miR-205 and showed that HOXD9 levels are inversely correlated with miR-205 levels in glioma tissues
Summary
Epigenetic, and transcriptomic testing suggested that gliomas can develop from multiple classes of cells in the central nervous system. Response to therapy is dependent on multiple factors, including tumor growth rate and invasiveness, and there is an urgent need for therapies directed against newly discovered molecular targets, those involved in epithelial–mesenchymal transition (EMT). EMT plays a fundamental role in promoting the invasion and metastasis of gliomas since it is a complex, multistage process, whereby cells lose their adhesive properties and become more motile and invasive [4]. Recent evidence has identified that multiple miRNAs are involved in regulating EMT [6,7]. MiRNAs play critical roles in several biological processes, including EMT and cancer development, making them viable targets for glioma intervention [8]
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