Abstract
BackgroundmiR-198 is involved in the formation, migration, invasion, and metastasis of various malignant cancers. However, the function and mechanism of action of miR-198 in the tumorigenesis of renal cell carcinoma (RCC) remain elusive. Here, we aimed to explore the role of miR198 in RCC.MethodsImmunohistochemistry was performed to estimate the level of survivin in RCC sections. Quantitative real-time polymerase chain reaction was performed to determine the expression level of miR-198 in fresh RCC tissues. Furthermore, the target relationship between miR-198 and BIRC5 was predicted using the TargetScanHuman 7.2 database and verified via dual-luciferase reporter assay and western blotting. The effects of miR-198 on the viability, apoptosis, invasion, and migration of A498 and ACHN cells were studied using Cell Counting Kit-8, flow cytometry, transwell migration assay, and wound healing assay, respectively. Additionally, a xenograft nude mouse model was established to evaluate the effect of miR-198 on RCC tumorigenesis.ResultsThe expression levels of BIRC5 and miR-198 were respectively higher and lower in RCC tissues than those in normal adjacent tissues. Furthermore, miR-198 could inhibit luciferase activity and reduce the protein level of survivin without affecting the BIRC5 mRNA levels. miR-198 inhibited cell viability, migration, and invasion and promoted cell apoptosis; co-transfection with BIRC5 could rescue these effects. Moreover, miR-198 could repress tumor growth in the xenograft nude mouse model of RCC.ConclusionsOur study demonstrates that miR-198 suppresses RCC progression by targeting BIRC5.
Highlights
MiR-198 is involved in the formation, migration, invasion, and metastasis of various malignant cancers
These results indicate that miR-198 could bind to the 3′-UTR of Baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC5) in renal cell carcinoma (RCC)
We examined the expression of miR-198 in eight pairs of fresh RCC tissues and adjacent tissues using quantitative polymerase chain reaction (PCR)
Summary
MiR-198 is involved in the formation, migration, invasion, and metastasis of various malignant cancers. The function and mechanism of action of miR-198 in the tumorigenesis of renal cell carcinoma (RCC) remain elusive. We aimed to explore the role of miR198 in RCC. Renal cell carcinoma (RCC) is the most invasive and common renal cancer in adults. Only up to 10% of patients with RCC present with characteristic clinical symptoms due to the lack of early warning signs [1]. 30% of patients with RCC present with metastases at the time of diagnosis [2]. RCC is resistant to radiotherapy and chemotherapy, and early diagnosis and successful surgical resection can save the patient’s life [1]. The 5-year survival rate of patients with stage I, Yuan et al Cancer Cell Int (2021) 21:390
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