Abstract
In recent years, studies have found that miR-RNA plays a role in cell proliferation, differentiation, apoptosis and metabolism. Among them, miR-196a is closely related to cervical cancer. Therefore, this experiment investigates the effect of mir-196a expression on cervical cancer cells and related mechanisms. The expression level of miR-196a in the cervical cancer cell line was assayed with the RT-PCR method, and liposome transfection was used to investigate its up-regulation or down-regulation in cervical cancer cells. The CCK-8 method and flow cytometry were used to measure cervical cancer cell proliferation and apoptosis, while the Transwell assay was used to determine cell migration and invasion of each transfection group. Bioinformatics was used to predict the target gene of miR-196a, which was verified using dual luciferase report experiment and Western blot, and miR-196a was further transfected with si-LRIG3 to detect its reversal effect on miR-196a regulation. Inhibition of the expression of miR-196a significantly reduced the proliferation, migration and invasion of cervical cancer cells, and promoted their apoptosis. Results from dual luciferase assay showed that miR-196a and LRIG3 had direct targeting effects. Cell proliferation, migration and invasion were enhanced by a reduction in the expression level of LRIG3 protein after miR-196a inhibitor cells were transfected with si-LRIG3. The expression of miR-196a is up-regulated in cervical cancer, and it promotes the growth of cervical cancer by its targeting effect on LRIG3 expression, resulting in enhancement of the proliferation, migration and invasion ability of cervical cancer cells, and inhibition of apoptosis.
Highlights
Cervical cancer is one of the most common malignant tumors in women
Results miR-196a is up-regulated in cervical cancer cell lines Results from RT-PCR showed that the expression level of miR-196a in the four cervical cancer cell lines Hela, CaSki, HCC94 and C33A were significantly higher than those in normal cervical cells Ect1/E6E7, and it was most upregulated in Hela cells (p
The results showed that inhibition of the expression of LRIG3 led to marked enhancements of the proliferation, migration and invasion capabilities of cervical cancer cells when compared with the miR-196a inhibitor group (p
Summary
Cervical cancer is one of the most common malignant tumors in women. The global new incidence has exceeded 500,000 annually, with an estimate of 300,000 deaths. The risk factors for cervical cancer are human papillomavirus (HPV) infection, smoking and multiple pregnancies [1]. The main treatment for patients with early cervical cancer is radical hysterectomy, and for patients with local progression or advanced stage, it is mainly radiotherapy or chemotherapy. Due to tumor metastasis or drug resistance of tumor cells, the 5-year survival of cervical cancer patients is not high. It is very important to identify new molecular markers that can be used for early diagnosis and treatment of cervical cancer targets, to improve the survival of cervical cancer patients. The occurrence of cervical cancer is a complex process involving the dysregulation of multiple gene expressions. With advancements in molecular biology, some progress has been made on the molecular mechanism of cervical cancer, but there are still many unclear issues
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