Abstract
The aim of this research was to assess the function of microribonucleic acid (miR)-195 in the apoptosis and proliferation of oral squamous cell carcinoma (OSCC) cells as well as its action mechanism. The downstream target protein of miR-195 was predicted using the biological software. A quantitative polymerase chain reaction (qPCR) was implemented to examine the changes in expressions of miR-195 and its target protein toll-like receptor 4 (TLR4) in OSCC cell lines (TSCCA, Tca8223, Tb3.1, and CAL-27) and normal adult human gingival fibroblasts (HGFs), and the relation between their expressions was assessed. The expressions of phosphorylated proteins in nuclear factor-κB (NF-κB) pathway were determined through western blotting. miR-195 was expressed at a noticeably lower level in four OSCC cells than in HGFs, and the lowest level appeared in CAL-27 cells. Compared with miR-195 control, the miR-195 mimic could obviously raise the expression of miR-195. In CAL-27 cells with high expression of miR-195, the proliferation was inhibited and the apoptosis was evidently enhanced. OSCC cells exhibited evidently reduced protein and mRNA expression of TLR4, and miR-195 expression was inversely associated with TLR4 expression. It was uncovered from the dual-luciferase reporter assay that cells with wild-type TLR4 had prominently weakened luciferase activity relative to cells with mutant-type TLR4, revealing that the direct target of miR-195 is TLR4. The NF-κB pathway was impeded in cells that lowly expressed TLR4. miR-195 blocks the NF-κB pathway via inhibiting the expression of TLR4 in OSCC cells, thereby exerting an antitumor effect.
Highlights
As a malignancy of the oral cavity, oral squamous cell carcinoma (OSCC) has a high relapse rate in the clinic, and the five-year survival rate in patients is below 50% [1]
In the past three decades, the five-year survival rate of OSCC patients was below 50% owing to the high relapse and death rates in spite of the remarkable efficiency in surgery and chemoradiotherapy for primary tumors in the oral cavity [2, 3]. ere is a necessity to comprehend the molecular mechanisms by which OSCC cells proliferate, invade, and metastasize, which are the basis for relapse, so as to efficaciously treat OSCC
Cell Lines. e objects of this study included OSCC TSCCA, Tca8223, Tb3.1, and CAL-27 cells, and transfection with exogenous microribonucleic acid (miR)-195 sequences raised miR-195 expression levels in CAL-27 cells. e cells undergoing miR195 mimic transfection were included in the miR-195 mimic group, while those receiving miR-195 control transfection were enrolled in the miR-195 control group
Summary
As a malignancy of the oral cavity, oral squamous cell carcinoma (OSCC) has a high relapse rate in the clinic, and the five-year survival rate in patients is below 50% [1]. Microribonucleic acids (miRNAs) are formed during mRNA maturation and consist of about 22 bases, which can silence downstream target mRNAs, thereby inhibiting protein expression and affecting tumor biological processes [4–8]. As a transcription factor in cells, nuclear factor-κB (NFκB) is mainly involved in the inflammatory response, and it can promote cell function and gene expression after activation [19]. Rough such a dynamic mechanism, the genes are regulated via activating transcription factors, such as NFκB [20]. E aim of the current research was to explore the effects of miR-195 on the apoptosis and proliferation of OSCC cells and to further investigate the potential underlying molecular mechanism
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
