MiR-193b-3p inhibits PLAU gene expression in patients with in-stent restenosis

Abstract

BackgroundThe coronary artery restenosis is a main problem in patients undergoing the stent implantation and angioplasty. The neointimal hyperplasia is one of the major causes for re-narrowing of blood vessels. Based on the prediction data, the aim of this study was to evaluate the PBMC miR-193b-3p and PLAU expression levels in patients with the stenosis of coronary arteries. MethodsA total of sixty three individuals undergoing coronary artery angiography including In-stent restenosis (n = 21, restenosis>70%), Stent non-restenosis (n = 21, stenosis<70%) and control (n = 21, stenosis<5%) subjects (n = 21, stenosis<5%) were selected. The gene and microRNA expression levels were measured using RT-qPCR technique. The microRNA and gene predictions were performed on the bioinformatics databases and datasets. ResultsThe miR-193b-3p was predicted to suppress the PLAU gene. The PLAU/miR193b-3p expression ratio was significantly increased in patients with In-stent restenosis conversely with control and Stent non-restenosis groups (P < 0.05). The gene and mirRNA expression levels were not correlated to other study parameters. ConclusionThe findings might suggest that the increase of monocyte PLAU gene expression is probably under control of miR-193b-3p. However, more investigations are needed to support the results.