Abstract
Background The present study is aimed at exploring the specific expression of miR-193a-3p and the mechanism underlying miR-193a-3p-mediated mesenchymal transition (MT), invasion, and migration in glioma. Methods The gene expression profile datasets of GSE39486 and GSE25676 were downloaded from the National Center for Biotechnology (NCBI). Data regarding the expression of miR-193a-3p and survival curves were derived from Chinese Glioma Genome Atlas (CGGA). Online websites including miRWalk, DIANA, and starbase were employed to predict the target genes for miR-193a-3p. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed by the Omicsbean online software. Module analysis of the protein-protein interaction (PPI) networks was performed by the plug-in Molecular Complex Detection (MCODE), and the degrees of genes were calculated by CytoHubba plug-in of Cytoscape. Survival curves were based on the Gene Expression Profile Interaction Analysis (GEPIA). Transwell, wound healing, and Western blot experiments were performed to investigate the effects of miR-193a-3p and beta-transducin repeat containing E3 ubiquitin protein ligase (BTRC) on the invasion, migration, and MT of glioma. Results miR-193a-3p was highly expressed in glioma tissues and significantly correlated with poor survival in patients with glioma. The target genes for miR-193a-3p were involved in many cancer-related signaling pathways. The PPI showed 11 genes with both high degrees and MCODE scores in the network. Survival analysis demonstrated that the expression of BTRC was significantly correlated with the prognosis of patients with glioma. The results from the transwell, wound healing, and Western blot analyses suggested that miR-193a-3p promoted the invasion, migration, and MT of glioma cells, which could be reversed by BTRC. Conclusions miR-193a-3p was upregulated in patients with glioma and could affect the invasion, migration, and MT of glioma by regulating BTRC.
Highlights
Glioma is the most common malignancy in the central nervous system [1] and possesses poor prognosis and high recurrence rate [2]
Wound-healing experiments showed that miR-193a-3p promoted cell migration (Figure 4(g)). These results indicated that miR-193a-3p should play an oncogenic role in glioma, and its elevation promoted the cell invasion, migration, and mesenchymal transition (MT) in glioma cell lines
We proved that miR-193a-3p could bind to the 3′UTR of beta-transducin repeat containing E3 ubiquitin protein ligase (BTRC) and its overexpression resulted in increased N-cadherin but decreased T-cadherin and BTRC in glioma cells
Summary
Glioma is the most common malignancy in the central nervous system [1] and possesses poor prognosis and high recurrence rate [2]. Despite the rapid advances in surgery, chemotherapy, and radiation therapy during the last decades, the average survival time for patients with malignant glioma has not been improved significantly [3]. It is to investigate the prognostic biomarkers and find new treatments for glioma. Survival analysis demonstrated that the expression of BTRC was significantly correlated with the prognosis of patients with glioma. The results from the transwell, wound healing, and Western blot analyses suggested that miR-193a-3p promoted the invasion, migration, and MT of glioma cells, which could be reversed by BTRC. Conclusions. miR-193a-3p was upregulated in patients with glioma and could affect the invasion, migration, and MT of glioma by regulating BTRC
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