Abstract
Introduction To investigate the function of miR-190a-3p on the proliferation and migration of glioma. Methods Twenty glioma samples and 6 normal brain tissue samples were collected. Normal human glial cell line HEB and glioma cell lines were used for the experiments. We then used TargetScan to predict the target genes of miR-190a-3p. Dual-luciferase reporter assay was also used to validate. Results Combined with dual-luciferase reporter experiment, we finally verified that YOD1 was the aim, and it was low-expressed in glioma. Besides, a series of mechanism experiments then proved that miR-190a-3p negatively regulates YOD1 expression. Conclusions Our research was the first to demonstrate the promoting function of miR-190a-3p in the proliferation and migration of glioma and provided new views for the treatment of glioma. miR-190a-3p was expected to be a new target for molecular therapy of glioma.
Highlights
To investigate the function of miR-190a-3p on the proliferation and migration of glioma
The therapy of glioma mainly rely on the surgery-assisted radiotherapy and chemotherapy, and the patients who suffer from these treatments often have poor prognosis and are very prone to relapse [4]
On a global scale, the treatment of this disease is still based on traditional treatment, such as surgery-assisted radiotherapy or chemotherapy
Summary
Glioma is one of the most usual primary intracranial tumors, accounting for nearly 50% of all primary intracranial tumors [1]. MiRNAs regulate the mRNA expression via combining with the target mRNA [5] They participate in a series of biological processes, for example, cell propagation and differentiation, stress, and apoptosis, and have important significance in tumor research [6]. Ciafrè et al analyzed 245 miRNAs in 9 pairs of human glioma tissues and 10 glioma cell lines. They found that 20 miRNAs are expressed differently in glioma, of which 9 miRNA are upregulated; 11 miRNA are downregulated [7]. Computational and Mathematical Methods in Medicine miRNAs may be involved in regulating malignant progression, angiogenesis, growth, recurrence, and migration of glioma [9,10,11,12]. We confirmed that miR-190a3p promoted the proliferation and migration of glioma by inhibiting the expression of YOD1, which provided new ideas for the therapy of glioma
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Computational and Mathematical Methods in Medicine
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
