MiR-181b as a Potential Molecular Target for Anticancer Therapy of Gastric Neoplasms

Jian-Xin Guo,Guang-Bo Yuan,Jun Chen,Xiao-Chun Chen,Peng-Rong Lou,Qing-Song Tao

doi:10.7314/apjcp.2012.13.5.2263

Abstract

MicroRNAs (miRNAs) play important roles in carcinogenesis. The aim of the present study was to explore the effects of miR-181b on gastric cancer. The expression level of miR-181b was quantified by qRT-PCR. MTT, flow cytometry and matrigel invasion assays were used to test proliferation, apoptosis and invasion of miR-181b stable transfected gastric cancer cells. miR-181b was aberrantly overexpressed in gastric cancer cells and primary gastric cancer tissues. Further experiments demonstrated inducible expression of miR-181b by Helicobacter pylori treatment. Cell proliferation, migration and invasion in the gastric cancer cells were significantly increased after miR-181b transfection and apoptotic cells were also increased. Furthermore, overexpression of miR-181b downregulated the protein level of tissue inhibitor of metalloproteinase 3 (TIMP3). The upregulation of miR-181b may play an important role in the progress of gastric cancer and miR-181b maybe a potential molecular target for anticancer therapeutics of gastric cancer.

Highlights

MicroRNAs are evolutionarily conserved small non-coding RNAs that regulate gene expression (Bartel, 2004)
Futhermore, we studied the roles of miR-181 in the invasion and metastasis, as well as the effect of anti-miR-181 on the apoptosis which shed a new light on the theraputic potential of miR-181b on gastric cancer
Results miR-181b was Aberrantly overexpressed in human gastric miR-181b oligonucleotide or the control was performed in 96-well plates in quadruplicate

Summary

Introduction

MicroRNAs (miRNAs) are evolutionarily conserved small non-coding RNAs that regulate gene expression (Bartel, 2004). We selected human gastric epithelial cell line GES-1, gastric carcinoma AGS and undifferentiated gastric carcinoma HGC-27 to define the expression of miR-181b. Cells were plated into a 96-well microplate and incubated with determining the expression of miR-181 family in a panel of human gastric cell lines by real-time PCR.

Results

Conclusion