MiR-17 enhances proliferation and migration and inhibits apoptosis in glioma cells by regulating SASH1 expression

Abstract

Background: To investigate the mechanisms underlying the regulation of human glioma cell growth, proliferation, and apoptosis by microRNA-17 (miR-17) to provide targets for novel human glioma therapies. Methods: The expression of miR-17 in human glioma tissues and cell lines was detected using real-time quantitative PCR (qRT-PCR). A miR-17 inhibitor was transfected into the U251 human glioma cell line using liposomes, and changes in miR-17 expression were detected using qRT-PCR. The effects of miR-17 on numerous biological characteristics of U251 cells, including viability, proliferation, apoptosis, and migration, were analyzed using MTT, flow cytometry, and Transwell migration chamber assays. A luciferase reporter gene system was used to validate SAM- and SH3-domain containing 1 ( SASH1 ) as a true target of miR-17. The effects of miR-17 on SASH1 protein expression were determined using western blotting. Results: qRT-PCR results showed that compared with adjacent tissues, miR-17 expression was significantly increased in human glioma tissues and cells lines (P Conclusions: miR-17 promotes viability, proliferation, and migration and inhibits apoptosis in U251 glioma cells, and these mechanisms could be mediated by the regulation of SASH1 gene expression.