Abstract
BackgroundAnaplastic thyroid cancer (ATC) is considered to be a rare type of thyroid cancer but takes up the most important proportion of thyroid cancer-related deaths. Therefore, the development of molecular targeted therapy is an exciting strategy in the management of ATC.MethodsmiR-155 and SOCS1 expression were measured by qRT-PCR as well as western blot analysis. 8305c and FRO cells were transfected and cultured for apoptosis assays, transwell, MTT on miR-155 or SOCS1 suppression and overexpression. Dual-luciferase reporter assays and SOCS1 restoration experimentswas implemented for define the relation between SOCS1 and miR-155. In addition, the correlation between miR-155 expression and patients’ clinicopathological features were also explored.ResultsAberrant miR-155 and SOCS1 expression and inverse correlation were found in ATC samples. In addition, it indicated that miR-155 expression correlated with cervical metastasis as well as extrathyroidal invasion. Moreover, we demonstrated that miR-155 inhibited 8305c and FRO cells apoptosis, promoted proliferation, invasion and migration. Furthermore, miR-155 inhibition was associated with a significant overexpression of SOCS1. Additionally, luciferase reporter assays presented that miR-155 could bind to SOCS1 3′-UTR, influencing its stability negatively and finally lowering SOCS1 levels. Moreover, it was illustrated that the impacts of miR-155 suppression were reversed by the inhibition of SOCS1 on cell proliferation, apoptosis as well as invasion.ConclusionsAberrant miR-155/SOCS1 expression has been included in ATC progression: miR-155 overexpression leads to SOCS1 suppression and develops ATC progression. Thus, miR-155 has been considered to be an underlying therapeutic target for ATC.
Highlights
Anaplastic thyroid cancer (ATC) is considered to be a rare type of thyroid cancer but takes up the most important proportion of thyroid cancer-related deaths
Merkel et al found that miR-155 downregulated SOCS1 to promote anaplastic large cell lymphoma [21]
Overexpression of miR-155 in human ATC tissues Adjacent non-tumor tissue samples as well as 31 paired ATC were tested by Quantitative real-time polymerase chain reaction (qRT-PCR) for the purpose of determining the expression of miR-155 in ATCs
Summary
Anaplastic thyroid cancer (ATC) is considered to be a rare type of thyroid cancer but takes up the most important proportion of thyroid cancer-related deaths. The development of molecular targeted therapy is an exciting strategy in the management of ATC. Thyroid cancer is considered to be the most common endocrine tumor [1]. Anaplastic thyroid cancer (ATC) is considered to be the rare subtype of thyroid cancer [2] but takes up the important proportion of thyroid cancer-related deaths [3, 4]. Zhang et al BMC Cancer (2019) 19:1093 defined the tumor-promoting function of miR-155/SOCS1 pathway in lung cancer progression [20]. We validated the regulatory relation between SOCS1 and miR-155 in ATCs. The miR155 might be an effective marker for predicting prognosis
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