MiR-150-5p suppresses tumor progression by targeting VEGFA in colorectal cancer.

Xiaoxiang Chen,Xueni Xu,Yuqin Pan,Bangshun He,Huiling Sun,Shukui Wang,Kaixuan Zeng,Mu Xu,Bei Pan,Xiangxiang Liu

doi:10.18632/aging.101656

Abstract

MicroRNA-150-5p (miR-150-5p) has been implicated in tumor initiation and progression in a variety of cancers. However, its roles in colorectal cancer (CRC) remain largely unknown. In our study, a decreased miR-150-5p expression in CRC tissues was found to be associated with poor overall survival. Moreover, miR-150-5p inhibited CRC cell proliferation, migration, invasion and angiogenesis in vitro and in vivo, and its inhibitory effect could be reversed by transfection of vascular epithelial growth factor A (VEGFA) expression plasmid. Lastly, we demonstrated that miR-150-5p inactivated VEGFA/VEGFR2 and the downstream Akt/mTOR signaling pathway in CRC. Based on these results, we conclude that miR-150-5p may function as a tumor suppressor in CRC, and miR-150-5p/VEGFA axis may be a potential therapeutic target candidate in CRC treatment.

Highlights

Colorectal cancer (CRC) is one of the most-prevalent malignancies and leading causes of cancer-related death in the world [1]
The results showed that miR-150-5p was significantly downregulated in colorectal cancer (CRC) tissues compared with that in matched adjacent normal tissues (ANTs) (Fig. 1B)
We sought to detect an association between miR-150-5p and a clinical outcome in CRC and showed that miR150-5p was closely associated with TNM stage, distant metastasis and overall survival

Summary

Introduction

Colorectal cancer (CRC) is one of the most-prevalent malignancies and leading causes of cancer-related death in the world [1]. Recent increasing evidence has confirmed that dysregulated miRNAs play key roles in multiple biological processes in CRC [6,7,8], including cell proliferation, drug resistance, apoptosis, metastasis and angiogenesis and have been identified as potential diagnostic, prognostic and therapeutic biomarkers in CRC diagnosis and treatment. MiR-150-5p, a cancer-related miRNA, has been reported to be aberrantly expressed in various cancers [9, 10]. Zhang Z, et al [11] have reported that miR-150 is increased in cervical cancer and promotes cell proliferation, migration and invasion by targeting PDCD4. Zhang L, et al [12] have reported that miR150 is upregulated in non-small cell lung cancer (NSCLC) cells and significantly associated with survival, and increased expression of miR-150 promotes NSCLC cell proliferation and migration. The potential mechanisms of miR-150-5p regulation of CRC progression remain unclear

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Results

Conclusion