Abstract
Glioma has been considered as one of the most prevalent and common malignancy of the nervous system; however, the underlying mechanisms that are responsible for the occurrence and development of glioma still remain largely unknown. Amounting evidence highlights the critical regulatory function of miRNAs in carcinogenesis. Here, we showed that the expression of miR-150-3p was significantly decreased in glioma tissues and cell lines. Suppressed expression of miR-150-3p was associated with the lymph node metastasis of the glioma patients. Overexpression of miR-150-3p significantly inhibited the proliferation of glioma cells. Molecular study uncovered that the transcription factor specificity protein 1 (SP1) was identified as one of the targets of miR-150-3p. Highly expressed miR-150-3p in glioma cells significantly decreased both the mRNA and protein levels of SP1. Consistently, the abundance of phosphatase and tension homolog deleted on chromosome ten (PTEN), a negative downstream target of SP1, was increased with the ectopic miR-150-3p. Collectively, these results suggested that miR-150-3p suppressed the growth of glioma cells partially via regulating SP1 and possibly PTEN.
Highlights
Glioma is the most common and malignant brain tumor, which accounts for approximately 80% of the brain carcinogenesis [1,2]
To detect the expression of miR-150-3p in glioma, room temperature (RT)-quantitative PCR (qPCR) analysis was performed with glioma tissues and normal brain tissues
The expression of miR-150-3p in glioma cell lines including Uppsala 87 Malignant Glioma (U87-MG), U251, A172, SWO-38, and Suzhou Human Glioma-44 (SHG-44) was measured and the data showed that the level of miR-150-3p was significantly decreased in all the above glioma cell lines compared with that of the normal astrocytes cells NHA (Figure 1B)
Summary
Glioma is the most common and malignant brain tumor, which accounts for approximately 80% of the brain carcinogenesis [1,2]. Due to the basic function of miRNAs in regulating gene expression, miRNAs are involved in a broad range of physiological processes, including cell proliferation, differentiation, stress condition, and tumorigenesis [13]. MiRNA-mediated resistance to chemotherapy has been observed in a variety of cancers [14,15,16,17,18,19]. These studies indicated the critical roles of miRNAs in the initiation and progression of cancers. The result showed that the expression of miR-150-3p was significantly decreased in glioma tissues (Supplementary Table S1), the potential function of miR-150-3p in glioma still remains unknown
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