Abstract
Osteosarcoma (OS) is the most common type of primary malignant bone tumor and mainly occurs in children and adolescent. Because of its early migration and invasion, OS has a poor prognosis. It has been reported that mircoRNAs (miRNAs) play a crucial role in the occurrence and development of multiple tumors. In this study, we identified the aberrant-expression of miR-143-3p in osteosarcoma and examined the role of miR-143-3p in OS development. Further, we searched the miR-143-3p target gene and verified its accuracy by luciferase experiments. Finally, we explored the relationship between miR-143-3p and FOS-Like antigen 2 (FOSL2). Our data indicated that miR-143-3p expression was substantially lower in OS tissues and cell-line compared with normal tissues, and was lower in patients with poor prognosis. In addition miR-143-3p inhibited OS cell proliferation and metastasis while promoting apoptosis. We next showed that FOSL2 was directly targeted by miR-143-3p and could reverse the inhibition caused by miR-143-3p. Finally, we found FOSL2 expression in OS cells was significantly higher compared with normal cells and negatively correlated with miR-143-3p. Thus, miR-143-3p directly and negatively targets FOSL2 to affect OS characteristics. This provides a new target for the treatment of OS and deserves further study.
Highlights
Osteosarcoma (OS) is a common primary malignant bone tumor occurring in adolescents and children especially in those under 20 years of age[1]
We investigated the relationship between miR-143-3p and its target gene FOS-like antigen 2 (FOSL2) in the regulation of OS
We collected OS tissues and adjacent normal tissues from 20 tumor cases, and measured the expression of miR-143-3p in each specimen. qRT-PCR analysis showed that the expression of miR-143-3p was significantly lower in OS cells than in Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei Province, P
Summary
Osteosarcoma (OS) is a common primary malignant bone tumor occurring in adolescents and children especially in those under 20 years of age[1]. The tumor cells can break through the cortex and medullary cavity and transfer through the blood to other tissues, especially the lung[4,5]. The metastasis of OS is still the main factor affecting the prognosis of OS patients. As a regulator of mRNA8, it regulates the entire process of cell proliferation, gene expression and even ontogenesis[9]. It has been reported that abnormal expression of miRNAs is found in a large number of human tumors including renal carcinoma, colon cancer, lung cancer, glioblastoma and gastric cancer[6,10,11]. Recent studies have revealed that many miRNAs are expressed abnormally in OS cells and play a role as oncogenes or anti-oncogenes in the occurrence and development of OS12,13. We investigated the relationship between miR-143-3p and its target gene FOS-like antigen 2 (FOSL2) in the regulation of OS
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