MiR-132 serves as a diagnostic biomarker in gestational diabetes mellitus and its regulatory effect on trophoblast cell viability

Xuegui Zhou,Xiaoxia Zheng,Cuiping Xiang

doi:10.1186/s13000-019-0899-9

Abstract

BackgroundGestational diabetes mellitus (GDM) leads to poor pregnancy outcomes. Strategies that improve trophoblast cell function are important methods for GDM treatment. This study aimed to investigate the expression and diagnostic potential of microRNA-132 (miR-132) in GDM patients, and further analyzed the effects of miR-132 on HTR-8/SVneo cell proliferation.MethodsQuantitative real-time PCR was applied to estimate the expression of miR-132. A receiver operating characteristics curve (ROC) analysis was performed to evaluate the diagnostic value of serum miR-132 in GDM patients. In vitro regulation of miR-132 in trophoblast cell HTR-8/SVneo was achieved by cell transfection, and the effects of miR-132 on cell proliferation were assessed using CCK-8 assay.ResultsExpression of miR-132 was decreased in serum and placenta tissues in GDM patients compared with the healthy women. A negative correlation was found between the serum miR-132 levels and fasting blood glucose of the GDM patients. A ROC curve shown the serum miR-132 had considerable diagnostic accuracy with an area under the curve (AUC) of 0.898. High glucose (HG) treatment induced an inhibition in HTR-8/SVneo cell proliferation and the expression of miR-132. The overexpression of miR-132 in HTR-8/SVneo cells could markedly rescued the HG - induced suppressed cell proliferation.ConclusionAll the data of this study revealed the reduced expression of miR-132 in serum and placenta tissues of GDM, and serum miR-132 serves a candidate biomarker in the diagnosis of GDM. miR-132 may act a protective role against GDM via enhancing the trophoblast cell proliferation.

Highlights

Gestational diabetes mellitus (GDM) is a special kind of diabetes mellitus, which first occurs in pregnancy with impaired glucose tolerance [1]
Increased blood glucose is a characteristic of GDM, and the fasting blood glucose was as expected elevated in the GDM patients in this study
According to Cell Counting Kit-8 (CCK-8) assay, we found that the overexpression of miR-132 could promote the trophoblast cell proliferation, while the knockdown of miR-132 resulted in the inhibited cell proliferation ability

Summary

Introduction

Gestational diabetes mellitus (GDM) is a special kind of diabetes mellitus, which first occurs in pregnancy with impaired glucose tolerance [1]. GDM induces numerous complications and leads to poor pregnancy outcomes, including abortion, fetal malformation, fetal growth restriction, premature rupture of membrane and macrosomia [3]. Similar to general diabetes mellitus, hyperglycemia and insulin resistance are the characteristics of GDM patients [4]. GDM has great potential to progress to type 2 diabetes mellitus (T2DM) if no any lifestyle, dietary or pharmacological intervention are administrated [5]. Early diagnosis is very important for the women with GDM. Gestational diabetes mellitus (GDM) leads to poor pregnancy outcomes. Strategies that improve trophoblast cell function are important methods for GDM treatment. This study aimed to investigate the expression and diagnostic potential of microRNA-132 (miR-132) in GDM patients, and further analyzed the effects of miR-132 on HTR-8/SVneo cell proliferation

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