MiR-132 Inhibits Tumor Metastasis

Abstract

It has been shown that some miRNAs are related with tumor invasion and metastasis directly or indirectly.Our aim is to find a specific miRNA which plays an essential role in tumor metastasis.Although it has been shown that miR-132 was associated with blood vessel growth,neural development and differentiation,and inflammation,relationship between miR-132 and tumor metastasis was not studied in any research.In order to identify the effect of miR-132 on tumor metastasis,the migration ability in vitro was detected on breast cancer cell line MDA-MB-231 cells transducted with miR-132(miR-132 group) and or mock miRNA(control group) by transwell assay.The results demonstrated significantly lower migration ratio in miR-132 case compared to that in control case,indicating inhibition effects on cancer migration of miR-132.To clarify the inhibition mechanism by which miR-132 inhibits cancer metastasis,target genes of miR-132 were screened and identified.They are CHIP(STUB1),G3BP1 and G3BP2.The expression levels of these 3 genes in MCF7 cells(metastasis cell line) and MDA-MB-231 cells with or without transduction of miR-132 or mock miRNA were detected by PCR and Real-time PCR.Two key genes,G3BP1/G3BP2,were founded to be involved in the regulation of miR-132 to tumor metastasis,demonstrating that miR-132 could silence G3BP1/G3BP2,which resulted in the suppression of tumor metastasis.Our research suggests that miR-132 may be an important potential target used for inhibition of cancer metastasis and clinical therapy of cancer,and shed light on the suppression mechanisms of miR-132.