MiR-1236-3p inhibits invasion and metastasis in gastric cancer by targeting MTA2

Jia-Xiang An,Nuo-Ming Zhou,Shuai Shao,Chun-Dong Zhang,Ming-Hui Ma,Dong-Qiu Dai

doi:10.1186/s12935-018-0560-9

Jia-Xiang An, Nuo-Ming Zhou + Show 4 more

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https://doi.org/10.1186/s12935-018-0560-9

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Abstract

BackgroundMicroRNAs deregulation are common in human tumor progression. miR-1236-3p has been reported to function as tumor suppressor microRNA in various malignancies. The aim of this study was to demonstrate the downregulated expression of miR-1236-3p in gastric cancer (GC) tissues and cell lines, and clarify its biological function in GC.MethodsReal-time polymerase chain reaction was used to measure the mRNA level of miR-1236-3p in GC. Dual luciferase assay was used to demonstrate that MTA2 was one of the candidate target genes of miR-1236-3p. Western blots were utilized to detect the protein levels. Cell function assays were also performed to determine the function of miR-1236-3p in GC.ResultsmiR-1236-3p expression, which was associated with lymph node metastasis, differentiation and clinical stage, was significantly reduced in GC tissues and cell lines. miR-1236-3p over-expression could inhibit GC cell proliferation, migration and invasion, and inhibition of miR-1236-3p expression had opposite effects. Furthermore, we demonstrated that MTA2 was a candidate target of miR-1236-3p, and miR-1236-3p over-expression significantly inhibited the process of epithelial–mesenchymal transition. We also found that miR-1236-3p could suppress the PI3K/Akt signaling pathway in GC cells.ConclusionsOur results suggest that miR-1236-3p functions as a tumor suppressor in GC and could be a promising therapeutic target for GC.

Highlights

MicroRNAs deregulation are common in human tumor progression. miR-1236-3p has been reported to function as tumor suppressor microRNA in various malignancies
Results miR‐1236‐3p is downregulated in gastric cancer (GC) cell lines and specimens To examine miR-1236-3p expression in GC cells, we measured the levels of miR-1236-3p in three GC cell lines, MKN-45, SGC-7901, and MGC-803, as well as a normal human gastric cell line, GES-1
The results indicated that the expression of miR-1236-3p was downregulated in MKN-45, SGC-7901, and MGC-803 cells compared with expression in GES-1 cells (Fig. 1a)

Summary

Introduction

MicroRNAs deregulation are common in human tumor progression. miR-1236-3p has been reported to function as tumor suppressor microRNA in various malignancies. The aim of this study was to demonstrate the down‐ regulated expression of miR-1236-3p in gastric cancer (GC) tissues and cell lines, and clarify its biological function in GC. MicroRNAs (miRNAs) are a class of small, non-coding RNA species, approximately 19–25 nucleotides in length. They can bind to the 3′-untranslated region (UTR) of the target mRNA and promote or inhibit the expression of target genes at the post-transcriptional level [7, 8]. Studies have shown that many miRNAs function as tumor suppressors or oncogenes in cancer cells, and participate in various biological processes such as proliferation, differentiation, apoptosis, and metastasis [10, 11]. Dysregulation of numerous miRNAs, such as mir-122, mir-26a, and mir-200c, has already been identified in GC [12,13,14], which suggests

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